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Analysis of trapping and streaming in an ultrasoundactuated multi-well microplate for single-cell studies

Ohlin, Mathias (author)
KTH,Tillämpad fysik
Christakou, Athanasia E. (author)
KTH,Tillämpad fysik
Frisk, Thomas (author)
KTH,Tillämpad fysik
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Önfelt, Björn (author)
KTH,Tillämpad fysik
Wiklund, Martin (author)
KTH,Tillämpad fysik
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 (creator_code:org_t)
Chemical and Biological Microsystems Society, 2012
2012
English.
In: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. - : Chemical and Biological Microsystems Society. - 9780979806452 ; , s. 497-499
  • Conference paper (peer-reviewed)
Abstract Subject headings
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  • The dynamics of the acoustic streaming and the acoustic positioning performance in an ultrasound-actuated multiwell microplate are investigated by two different ultrasonic frequency actuation schemes: Frequency-modulation and single- frequency actuation. Our results show a significant decrease in size of the field of view when using frequencymodulation compared to single-frequency actuation, which can be used for improving the scanning time for 3D highresolution confocal microscopy by almost one order of magnitude. Furthermore, in the ultrasound-actuated multi-well microplate the high-voltage acoustic streaming show a complex time and temperature dependence and could gain stability by the use of temperature control.

Subject headings

NATURVETENSKAP  -- Fysik -- Annan fysik (hsv//swe)
NATURAL SCIENCES  -- Physical Sciences -- Other Physics Topics (hsv//eng)

Keyword

Acoustic streaming
Microplate
Microscopy
Multi-well
Positioning
Ultrasound

Publication and Content Type

ref (subject category)
kon (subject category)

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