id:"swepub:oai:DiVA.org:kth-172260"
Search: id:"swepub:oai:DiVA.org:kth-172260" > Characterization of...
- 1 of 1
- Previous record
- Next record
- To hitlist
Characterization of motility and piliation in pathogenic Neisseria
-
- Eriksson, Jens (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
-
- Eriksson, Olaspers Sara (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
-
- Maudsdotter, Lisa (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
- show more...
- show less...
- (creator_code:org_t)
- 2015-04-30
- 2015
- English.
- In: BMC Microbiology. - : Springer Science and Business Media LLC. - 1471-2180. ; 15
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Background: The type IV pili (Tfp) of pathogenic Neisseria (i. e., N. gonorrhoeae and N. meningitidis) are essential for twitching motility. Tfp retraction, which is dependent on the ATPase PilT, generates the forces that move bacteria over surfaces. Neisseria motility has mainly been studied in N. gonorrhoeae whereas the motility of N. meningitidis has not yet been characterized. Results: In this work, we analyzed bacterial motility and monitored Tfp retraction using live- cell imaging of freely moving bacteria. We observed that N. meningitidis moved over surfaces at an approximate speed of 1.6 mu m/s, whereas N. gonorrhoeae moved with a lower speed (1.0 mu/s). An alignment of the meningococcal and gonococcal pilT promoters revealed a conserved single base pair variation in the -10 promoter element that influence PilT expression. By tracking mutants with altered pilT expression or pilE sequence, we concluded that the difference in motility speed was independent of both. Live-cell imaging using total internal reflection fluorescence microscopy demonstrated that N. gonorrhoeae more often moved with fewer visible retracting filaments when compared to N. meningitidis. Correspondingly, meningococci also displayed a higher level of piliation in transmission electron microscopy. Nevertheless, motile gonococci that had the same number of filaments as N. meningitidis still moved with a lower speed. Conclusions: These data reveal differences in both speed and piliation between the pathogenic Neisseria species during twitching motility, suggesting a difference in Tfp-dynamics.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Neisseria
- Type IV pili
- PilT
- Twitching motility
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- BMC Microbiology (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Eriksson, Jens
- Eriksson, Olaspe ...
- Maudsdotter, Lis ...
- Palm, Oskar
- Engman, Jakob
- Sarkissian, Tim
- show more...
- Aro, Helena
- Wallin, Mats
- Jonsson, Ann-Bet ...
- show less...
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- and Microbiology
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- Articles in the publication
- BMC Microbiology
- By the university
- Royal Institute of Technology
- Stockholm University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
