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A saposin-lipoprote...
A saposin-lipoprotein nanoparticle system for membrane proteins
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Frauenfeld, Jens (author)
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Loving, Robin (author)
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Armache, Jean-Paul (author)
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Sonnen, Andreas F-P (author)
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Guettou, Fatma (author)
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Moberg, Per (author)
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- Zhu, Lin (author)
- KTH,Hälso- och systemvetenskap
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- Jegerschöld, Caroline (author)
- KTH,Strukturell bioteknik
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Flayhan, Ali (author)
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Briggs, John A. G. (author)
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Garoff, Henrik (author)
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Low, Christian (author)
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Cheng, Yifan (author)
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Nordlund, Par (author)
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(creator_code:org_t)
- Nature Publishing Group, 2016
- 2016
- English.
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In: Nature Methods. - : Nature Publishing Group. - 1548-7091 .- 1548-7105. ; 13:4, s. 345-351
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- A limiting factor in membrane protein research is the ability to solubilize and stabilize such proteins. Detergents are used most often for solubilizing membrane proteins, but they are associated with protein instability and poor compatibility with structural and biophysical studies. Here we present a saposin-lipoprotein nanoparticle system, Salipro, which allows for the reconstitution of membrane proteins in a lipid environment that is stabilized by a scaffold of saposin proteins. We demonstrate the applicability of the method on two purified membrane protein complexes as well as by the direct solubilization and nanoparticle incorporation of a viral membrane protein complex from the virus membrane. Our approach facilitated high-resolution structural studies of the bacterial peptide transporter PeptT(S02) by single-particle cryo-electron microscopy (cryo-EM) and allowed us to stabilize the HIV envelope glycoprotein in a functional state.
Subject headings
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Frauenfeld, Jens
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Loving, Robin
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Armache, Jean-Pa ...
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Sonnen, Andreas ...
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Guettou, Fatma
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Moberg, Per
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show more...
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Zhu, Lin
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Jegerschöld, Car ...
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Flayhan, Ali
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Briggs, John A. ...
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Garoff, Henrik
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Low, Christian
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Cheng, Yifan
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Nordlund, Par
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
- Articles in the publication
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Nature Methods
- By the university
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Royal Institute of Technology