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Acoustic micro-vort...
Acoustic micro-vortexing of fluids, beads and cells in disposible microfluidic chips
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- Iranmanesh, Ida (författare)
- KTH,Tillämpad fysik
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- Ohlin, Mathias (författare)
- KTH,Tillämpad fysik
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- Ramachandraiah, Harisha (författare)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
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visa fler...
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- Russom, Aman (författare)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Wiklund, Martin (författare)
- KTH,Tillämpad fysik
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visa färre...
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(creator_code:org_t)
- Chemical and Biological Microsystems Society, 2015
- 2015
- Engelska.
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Ingår i: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806483 ; , s. 1005-1007
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- In this paper we demonstrate a multi-functional platform using ultrasound for vortexing of 20-μl volumes of different samples in polymer-based disposable chips. The method enables different vortexing functions such as mixing laminar flows, resuspension of a micro-pellet of magnetic beads as well as cell lysis for DNA extraction. The device consists of an inexpensive low-frequency, high power, horn-shaped langevin transducer which is typically used for cell disruption in larger volumes. By controlling the operating time of this device (from fractions of a second up to a minute) different functions can be achieved. In addition, to avoid the high-power-induced heating, a simple cooling system is used as a chip holder consisting of a PC fan-cooled aluminum heat sink. To demonstrate a sample preparation application, we perform on-chip cell lysis and DNA extraction.
Ämnesord
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
Nyckelord
- Cell lysis
- Microfluidic chip
- Mixing
- Ultrasound
- Vortexing
- Extraction
- Fluidic devices
- Laminar flow
- Ultrasonics
- Cell disruption
- Induced heating
- Langevin transducers
- Multi-functional
- Sample preparation
- Microfluidics
Publikations- och innehållstyp
- ref (ämneskategori)
- kon (ämneskategori)
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