id:"swepub:oai:DiVA.org:kth-19839"
Search: id:"swepub:oai:DiVA.org:kth-19839" > Influence of scale-...
- 1 of 1
- Previous record
- Next record
- To hitlist
Influence of scale-up on the quality of recombinant human growth hormone
- Bylund, F. (author)
- Castan, A. (author)
- Mikkola, R. (author)
- show more...
- Larsson, G. (author)
- show less...
- (creator_code:org_t)
- 2000
- 2000
- English.
- In: Biotechnology and Bioengineering. - 0006-3592 .- 1097-0290. ; 69:2, s. 119-128
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- The aerobic fed-batch production of recombinant human growth hormone (rhGH) by Escherichia coli was studied. The goal was to determine the production and protein degradation pattern of this product during fed-batch cultivation and to what extent scale differences depend on the presence of a fed-batch glucose feed zone. Results of laboratory bench-scale, scale-down (SDR), and industrial pilot-scale (3-m(3)) reactor production were compared. In addition to the parameters of product yield and quality, also cell yield, respiration, overflow, mixed acid fermentation, glucose concentration, and cell lysis were studied and compared. The results show that oxygen limitation following glucose overflow was the critical parameter and not the glucose overflow itself. This was verified by the pattern of byproduct formation where formate was the dominating factor and not acetic acid. A correlation between the accumulation of formate, the degree of heterogeneity, and cell lysis was also visualized when recombinant protein was expressed. The production pattern could be mimicked in the SDR reactor for all parameters, except for product quantity and quality, where 30% fewer rhGH-degraded forms were present and where about 80% higher total yield was achieved, resulting in 10% greater accumulation of properly formed rhGH monomer.
Keyword
- recombinant human growth hormone (rhGH)
- product quality
- Escherichia coli fed-batch
- substrate gradients
- scale-up
- scale-down reactor (SDR)
- gluconic acid fermentation
- fed-batch cultivations
- baker yeast production
- escherichia-coli
- experimental simulation
- saccharomyces-cerevisiae
- substrate oscillations
- oxygen profiles
- bioreactor
- protein
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Biotechnology and Bioengineering (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Bylund, F.
- Castan, A.
- Mikkola, R.
- Veide, Andres
- Larsson, G.
- Articles in the publication
- Biotechnology an ...
- By the university
- Royal Institute of Technology
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
