The function of two type II metacaspases in woody tissues of Populus trees

Bollhöner, Benjamin (author): Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)

Jokipii-Lukkari, Soile (author): Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC),Umeå University

Bygdell, Joakim (author): Umeå universitet,Kemiska institutionen,Umeå University

Stael, Simon (author)

Muniz, Luis (author): Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)

Van Breusegem, Frank (author)

Ezcurra, Ines (author): KTH,Industriell bioteknologi,Royal Institute of Technology (KTH)

Wingsle, Gunnar (author): Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology

Tuominen, Hannele (author): Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)

(creator_code:org_t)

2017-12-15
2018
English.
In: New Phytologist. - : Wiley. - 0028-646X .- 1469-8137. ; 217:4, s. 1551-1565

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Abstract Subject headings

Metacaspases (MCs) are cysteine proteases that are implicated in programmed cell death of plants. AtMC9 (Arabidopsis thaliana Metacaspase9) is a member of the Arabidopsis MC family that controls the rapid autolysis of the xylem vessel elements, but its downstream targets in xylem remain uncharacterized. PttMC13 and PttMC14 were identified as AtMC9 homologs in hybrid aspen (Populustremulaxtremuloides). A proteomic analysis was conducted in xylem tissues of transgenic hybrid aspen trees which carried either an overexpression or an RNA interference construct for PttMC13 and PttMC14. The proteomic analysis revealed modulation of levels of both previously known targets of metacaspases, such as Tudor staphylococcal nuclease, heat shock proteins and 14-3-3 proteins, as well as novel proteins, such as homologs of the PUTATIVE ASPARTIC PROTEASE3 (PASPA3) and the cysteine protease RD21 by PttMC13 and PttMC14. We identified here the pathways and processes that are modulated by PttMC13 and PttMC14 in xylem tissues. In particular, the results indicate involvement of PttMC13 and/or PttMC14 in downstream proteolytic processes and cell death of xylem elements. This work provides a valuable reference dataset on xylem-specific metacaspase functions for future functional and biochemical analyses.

LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

By the author/editor: Bollhöner, Benja ...; Jokipii-Lukkari, ...; Bygdell, Joakim; Stael, Simon; Adriasola, Mathi ...; Muniz, Luis; show more...; Van Breusegem, F ...; Ezcurra, Ines; Wingsle, Gunnar; Tuominen, Hannel ...; show less...

About the subject

AGRICULTURAL SCIENCES: AGRICULTURAL SCI ...; and Agricultural Bio ...; and Plant Biotechnol ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

By the university: Royal Institute of Technology; Umeå University; Swedish University of Agricultural Sciences

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