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Development of a Ba...
Development of a Bacterial Biosensor for Rapid Screening of Yeast p-Coumaric Acid Production
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Siedler, S. (author)
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- Khatri, Narendar K. (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Technical University of Denmark
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Zsohár, A. (author)
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Kjærbølling, I. (author)
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Vogt, M. (author)
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- Hammar, Petter (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
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Nielsen, C. F. (author)
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Marienhagen, J. (author)
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Sommer, M. O. A. (author)
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- Jönsson, Håkan, 1979- (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Technical University of Denmark
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(creator_code:org_t)
- 2017-06-21
- 2017
- English.
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In: ACS Synthetic Biology. - : American Chemical Society (ACS). - 2161-5063. ; 6:10, s. 1860-1869
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Transcription factor-based biosensors are used to identify producer strains, a critical bottleneck in cell factory engineering. Here, we address two challenges with this methodology: transplantation of heterologous transcriptional regulators into new hosts to generate functional biosensors and biosensing of the extracellular product concentration that accurately reflects the effective cell factory production capacity. We describe the effects of different translation initiation rates on the dynamic range of a p-coumaric acid biosensor based on the Bacillus subtilis transcriptional repressor PadR by varying its ribosomal binding site. Furthermore, we demonstrate the functionality of this p-coumaric acid biosensor in Escherichia coli and Corynebacterium glutamicum. Finally, we encapsulate yeast p-coumaric acid-producing cells with E. coli-biosensing cells in picoliter droplets and, in a microfluidic device, rapidly sort droplets containing yeast cells producing high amounts of extracellular p-coumaric acid using the fluorescent E. coli biosensor signal. As additional biosensors become available, such approaches will find broad applications for screening of an extracellular product.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
Keyword
- Para coumaric acid
- recombinant DNA
- bacterial protein
- propionic acid derivative
- trans-3-(4'-hydroxyphenyl)-2-propenoic acid
- transcription factor
- article
- bacillus subtilis
- bacterium culture
- binding site
- catalysis
- cell population
- cell selection
- coculture
- controlled study
- Corynebacterium glutamicum
- Escherichia coli
- feedback system
- fluorescence
- fungal cell culture
- gene repression
- Lactococcus lactis
- microbiological examination
- microencapsulation
- microfluidics
- mutagenesis
- nonhuman
- osmosis
- phenotype
- priority journal
- promoter region
- translation initiation
- water transport
- yeast
- yeast cell
- genetic procedures
- metabolism
- procedures
- Bacterial Proteins
- Biosensing Techniques
- Propionates
- Transcription Factors
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Siedler, S.
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Khatri, Narendar ...
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Zsohár, A.
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Kjærbølling, I.
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Vogt, M.
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Hammar, Petter
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show more...
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Nielsen, C. F.
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Marienhagen, J.
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Sommer, M. O. A.
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Jönsson, Håkan, ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
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and Other Industrial ...
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
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and Biocatalysis and ...
- Articles in the publication
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ACS Synthetic Bi ...
- By the university
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Royal Institute of Technology