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Deformation strain is the main physical driver for skeletal precursors to undergo osteogenesis in earlier stages of osteogenic cell maturation
- Ramani-Mohan, R. -K (författare)
- Schwedhelm, I. (författare)
-
- Wistrand, Anna Finne, 1976- (författare)
- KTH,Fiber- och polymerteknologi
- visa fler...
- Krug, M. (författare)
- Schwarz, T. (författare)
- Jakob, F. (författare)
- Walles, H. (författare)
- Hansmann, J. (författare)
- visa färre...
- (creator_code:org_t)
- 2017-11-10
- 2018
- Engelska.
- Ingår i: Journal of Tissue Engineering and Regenerative Medicine. - : John Wiley and Sons Ltd. - 1932-6254 .- 1932-7005. ; 12:3, s. e1474-e1479
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Mesenchymal stem cells play a major role during bone remodelling and are thus of high interest for tissue engineering and regenerative medicine applications. Mechanical stimuli, that is, deformation strain and interstitial fluid-flow-induced shear stress, promote osteogenic lineage commitment. However, the predominant physical stimulus that drives early osteogenic cell maturation is not clearly identified. The evaluation of each stimulus is challenging, as deformation and fluid-flow-induced shear stress interdepend. In this study, we developed a bioreactor that was used to culture mesenchymal stem cells harbouring a strain-responsive AP-1 luciferase reporter construct, on porous scaffolds. In addition to the reporter, mineralization and vitality of the cells was investigated by alizarin red staining and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. Quantification of the expression of genes associated to bone regeneration and bone remodelling was used to confirm alizarin red measurements. Controlled perfusion and deformation of the 3-dimensional scaffold facilitated the alteration of the expression of osteogenic markers, luciferase activity, and calcification. To isolate the specific impact of scaffold deformation, a computational model was developed to derive a perfusion flow profile that results in dynamic shear stress conditions present in periodically loaded scaffolds. In comparison to actually deformed scaffolds, a lower expression of all measured readout parameters indicated that deformation strain is the predominant stimulus for skeletal precursors to undergo osteogenesis in earlier stages of osteogenic cell maturation.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- bioreactor
- computational fluid dynamics
- mechanical strain
- mechanosensitive reporter gene constructs
- mesenchymal stem cells
- osteogenesis
- 3 (4
- 5 dimethyl 2 thiazolyl) 2
- 5 diphenyltetrazolium bromide
- alizarin red s
- alkaline phosphatase
- bone morphogenetic protein 2
- collagen type 1
- osteocalcin
- osteonectin
- osteopontin
- transcription factor AP 1
- transcription factor RUNX2
- Article
- bone deformation
- bone development
- bone mineralization
- bone regeneration
- bone remodeling
- bone stress
- cell culture
- cell differentiation
- cell lineage
- cell maturation
- controlled study
- enzyme activity
- gene expression
- human
- human cell
- luciferase assay
- mathematical model
- mesenchymal stem cell
- osteoblast
- porosity
- priority journal
- quantitative analysis
- shear stress
- stem cell
- stress
- three dimensional imaging
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Journal of Tissue Engineering and Regenerative Medicine (Sök värdpublikationen i LIBRIS)
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- Schwarz, T.
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- Walles, H.
- Hansmann, J.
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