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A systems-approach ...
A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein
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- Dusart, Philip (author)
- KTH,Science for Life Laboratory, SciLifeLab,Skolan för bioteknologi (BIO)
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- Fagerberg, Linn (author)
- KTH,Science for Life Laboratory, SciLifeLab,Skolan för bioteknologi (BIO)
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- Perisic, L. (author)
- Karolinska Institutet
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Civelek, M. (author)
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- Struck, Eike (author)
- KTH,Skolan för bioteknologi (BIO),Science for Life Laboratory, SciLifeLab
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- Hedin, U. (author)
- Karolinska Institutet
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- Uhlén, Mathias (author)
- KTH,Science for Life Laboratory, SciLifeLab,Skolan för bioteknologi (BIO)
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Trégouët, D. -A (author)
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Renné, T. (author)
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- Odeberg, Jacob (author)
- Karolinska Institutet,KTH,Science for Life Laboratory, SciLifeLab,Skolan för bioteknologi (BIO),Coagulation Unit, Centre for Hematology, Karolinska University Hospital, SE-171 76, Stockholm, Sweden
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- Butler, Lynn M. (author)
- Karolinska Institutet,KTH,Science for Life Laboratory, SciLifeLab,Skolan för bioteknologi (BIO),Clinical Chemistry and Blood Coagulation, Department of Molecular Medicine and Surgery, Karolinska Institute, SE-171 76, Stockholm, Sweden Institute for Clinical Chemistry and Laboratory Medicine, University Medical Centre Hamburg-Eppendorf, D-20246, Hamburg, Germany
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(creator_code:org_t)
- 2018-10-02
- 2018
- English.
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In: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 8:1
- Related links:
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The intermediate filament protein nestin is expressed during embryonic development, but considered largely restricted to areas of regeneration in the adult. Here, we perform a body-wide transcriptome and protein-profiling analysis to reveal that nestin is constitutively, and highly-selectively, expressed in adult human endothelial cells (EC), independent of proliferative status. Correspondingly, we demonstrate that it is not a marker for tumour EC in multiple malignancy types. Imaging of EC from different vascular beds reveals nestin subcellular distribution is shear-modulated. siRNA inhibition of nestin increases EC proliferation, and nestin expression is reduced in atherosclerotic plaque neovessels. eQTL analysis reveals an association between SNPs linked to cardiovascular disease and reduced aortic EC nestin mRNA expression. Our study challenges the dogma that nestin is a marker of proliferation, and provides insight into its regulation and function in EC. Furthermore, our systems-based approach can be applied to investigate body-wide expression profiles of any candidate protein.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Dusart, Philip
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Fagerberg, Linn
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Perisic, L.
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Civelek, M.
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Struck, Eike
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Hedin, U.
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show more...
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Uhlén, Mathias
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Trégouët, D. -A
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Renné, T.
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Odeberg, Jacob
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Butler, Lynn M.
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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Scientific Repor ...
- By the university
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Royal Institute of Technology
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Karolinska Institutet