Sökning: id:"swepub:oai:DiVA.org:kth-240204" > Influence of compos...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 04803naa a2200589 4500 | |
001 | oai:DiVA.org:kth-240204 | |
003 | SwePub | |
008 | 190111s2018 | |||||||||||000 ||eng| | |
009 | oai:DiVA.org:uu-358029 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2402042 URI |
024 | 7 | a https://doi.org/10.1007/s00726-018-2571-12 DOI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3580292 URI |
040 | a (SwePub)kthd (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Oroujeni, Maryam,c PhD,d 1982-u Uppsala universitet,Medicinsk strålningsvetenskap4 aut0 (Swepub:uu)maror883 |
245 | 1 0 | a Influence of composition of cysteine-containing peptide-based chelators on biodistribution of Tc-99m-labeled anti-EGFR affibody molecules |
264 | c 2018-05-04 | |
264 | 1 | b Springer,c 2018 |
338 | a print2 rdacarrier | |
500 | a QC 20190111 | |
520 | a Epidermal growth factor receptor (EGFR) is overexpressed in a number of cancers and is the molecular target for several anti-cancer therapeutics. Radionuclide molecular imaging of EGFR expression should enable personalization of anti-cancer treatment. Affibody molecule is a promising type of high-affinity imaging probes based on a non-immunoglobulin scaffold. A series of derivatives of the anti-EGFR affibody molecule ZEGFR:2377, having peptide-based cysteine-containing chelators for conjugation of Tc-99m, was designed and evaluated. It was found that glutamate-containing chelators Gly-Gly-Glu-Cys (GGEC), Gly-Glu-Glu-Cys (GEEC) and Glu-Glu-Glu-Cys (EEEC) provide the best labeling stability. The glutamate containing conjugates bound to EGFR-expressing cells specifically and with high affinity. Specific targeting of EGFR-expressing xenografts in mice was demonstrated. The number of glutamate residues in the chelator had strong influence on biodistribution of radiolabeled affibody molecules. Increase of glutamate content was associated with lower uptake in normal tissues. The Tc-99m-labeled variant containing the EEEC chelator provided the highest tumor-to-organ ratios. In conclusion, optimizing the composition of peptide-based chelators enhances contrast of imaging of EGFR-expression using affibody molecules. | |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Läkemedelskemi0 (SwePub)301032 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Medicinal Chemistry0 (SwePub)301032 hsv//eng |
653 | a Affibody molecules | |
653 | a EGFR | |
653 | a Tc-99m | |
653 | a Peptide-based chelators | |
653 | a Glutamate | |
700 | 1 | a Andersson, Ken G.u Uppsala universitet,Medicinsk strålningsvetenskap4 aut |
700 | 1 | a Steinhardt, Xeniau KTH,Proteinvetenskap,KTH Royal Inst Technol, Dept Prot Sci, Stockholm, Sweden.4 aut0 (Swepub:kth)u1izzc3b |
700 | 1 | a Altai, Mohamedu Uppsala universitet,Medicinsk strålningsvetenskap4 aut0 (Swepub:uu)mohal988 |
700 | 1 | a Orlova, Anna,d 1960-u Uppsala universitet,Theranostics4 aut0 (Swepub:uu)annaorlo |
700 | 1 | a Mitran, Bogdanu Uppsala universitet,Theranostics4 aut0 (Swepub:uu)mitbo652 |
700 | 1 | a Vorobyeva, Anzhelikau Uppsala universitet,Medicinsk strålningsvetenskap4 aut0 (Swepub:uu)anzvo555 |
700 | 1 | a Garousi, Javadu Uppsala universitet,Medicinsk strålningsvetenskap4 aut0 (Swepub:uu)javga723 |
700 | 1 | a Tolmachev, Vladimiru Uppsala universitet,Medicinsk strålningsvetenskap4 aut0 (Swepub:uu)vladtolm |
700 | 1 | a Löfblom, Johnu KTH,Proteinvetenskap,KTH Royal Inst Technol, Dept Prot Sci, Stockholm, Sweden.4 aut0 (Swepub:kth)u1z7cgkl |
710 | 2 | a Uppsala universitetb Medicinsk strålningsvetenskap4 org |
773 | 0 | t Amino Acidsd : Springerg 50:8, s. 981-994q 50:8<981-994x 0939-4451x 1438-2199 |
856 | 4 | u https://link.springer.com/content/pdf/10.1007/s00726-018-2571-1.pdf |
856 | 4 | u https://doi.org/10.1007/s00726-018-2571-1y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1241515/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-240204 |
856 | 4 8 | u https://doi.org/10.1007/s00726-018-2571-1 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-358029 |
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