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Improved contrast o...
Improved contrast of affibody-mediated imaging of HER3 expression in mouse xenograft model through co-injection of a trivalent affibody for in vivo blocking of hepatic uptake
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- Rosestedt, Maria (författare)
- Uppsala universitet,Theranostics
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- Andersson, Ken G. (författare)
- KTH Royal Inst Technol, Dept Prot Sci, Stockholm, Sweden
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- Rinne, Sara S. (författare)
- Uppsala universitet,Theranostics,KTH Royal Inst Technol, Dept Prot Sci, Stockholm, Sweden
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- Leitao, Charles Dahlsson (författare)
- KTH,Proteinvetenskap,KTH Royal Inst Technol, Dept Prot Sci, Stockholm, Sweden
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- Mitran, Bogdan (författare)
- Uppsala universitet,Theranostics
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- Vorobyeva, Anzhelika (författare)
- Uppsala universitet,Medicinsk strålningsvetenskap
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- Ståhl, Stefan (författare)
- KTH,Albanova VinnExcellence Center for Protein Technology, ProNova
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- Löfblom, John (författare)
- KTH,Proteinvetenskap,KTH Royal Inst Technol, Dept Prot Sci, Stockholm, Sweden
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- Tolmachev, Vladimir (författare)
- Uppsala universitet,Medicinsk strålningsvetenskap
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- Orlova, Anna (författare)
- Uppsala universitet,Theranostics,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- 2019-05-01
- 2019
- Engelska.
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Ingår i: Scientific Reports. - : Springer Nature. - 2045-2322. ; 9
- Relaterad länk:
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https://doi.org/10.1...
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https://www.nature.c...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Human epidermal growth factor receptor type 3 (HER3) plays a crucial role in the progression of many cancer types. In vivo radionuclide imaging could be a reliable method for repetitive detection of HER3-expression in tumors. The main challenge of HER3-imaging is the low expression in tumors together with endogenous receptor expression in normal tissues, particularly the liver. A HER3-targeting affibody molecule labeled with radiocobalt via a NOTA chelator [Co-57]Co-NOTA-Z(08699) has demonstrated the most favorable biodistribution profile with the lowest unspecific hepatic uptake and high activity uptake in tumors. We hypothesized that specific uptake of labeled affibody monomer might be selectively blocked in the liver but not in tumors by a co-injection of non-labeled corresponding trivalent affibody (Z(08699))(3). Biodistribution of [Co-57]Co-NOTA-Z(08699) and [In-111]ln-DOTA-(Z(08699))(3) was studied in BxPC-3 xenografted mice. [Co-57]Co-NOTA-Z(08699) was co-injected with unlabeled trivalent affibody DOTA-(Z(08699))(3) at different monomer:trimer molar ratios. HER3-expression in xenografts was imaged using [Co-57]Co-NOTA-Z(08699) and [Co-57]Co-NOTA-Z(08699): DOTA-(Z(08699))(3). Hepatic activity uptake of [Co-57] Co-NOTA-Z(08699): DOTA-(Z(08699))(3) decreased with increasing monomer:trimer molar ratio. The tumor activity uptake and tumor-to-liver ratios were the highest for the 1:3 ratio. SPECT/CT images confirmed the biodistribution data. Imaging of HER3 expression can be improved by co-injection of a radiolabeled monomeric affi body-based imaging probe together with a trivalent affibody.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Radiologi och bildbehandling (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Radiology, Nuclear Medicine and Medical Imaging (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Rosestedt, Maria
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Andersson, Ken G ...
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Rinne, Sara S.
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Leitao, Charles ...
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Mitran, Bogdan
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Vorobyeva, Anzhe ...
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visa fler...
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Ståhl, Stefan
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Löfblom, John
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Tolmachev, Vladi ...
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Orlova, Anna
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Radiologi och bi ...
- Artiklar i publikationen
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Scientific Repor ...
- Av lärosätet
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Kungliga Tekniska Högskolan
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Uppsala universitet