Search: id:"swepub:oai:DiVA.org:kth-277660" >
Electrochemical Det...
Electrochemical Detection of Genomic DNA Utilizing Recombinase Polymerase Amplification and Stem-Loop Probe
-
- Khaliliazar, Shirin (author)
- KTH,Fiberteknologi
-
- Ouyang, Liangqi (author)
- KTH,Fiberteknologi
-
- Piper, Andrew (author)
- KTH,Fiberteknologi
-
show more...
-
- Chondrogiannis, Georgios (author)
- KTH,Fiberteknologi
-
- Hanze, Martin (author)
- KTH,Fiberteknologi
-
- Herland, Anna (author)
- Karolinska Institutet,KTH,Mikro- och nanosystemteknik
-
- Hamedi, Mahiar (author)
- KTH,Fiberteknologi
-
show less...
-
(creator_code:org_t)
- 2020-05-20
- 2020
- English.
-
In: ACS Omega. - : AMER CHEMICAL SOC. - 2470-1343. ; 5:21, s. 12103-12109
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://pubs.acs.org...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- Nucleic acid tests integrated into digital point-of-care (POC) diagnostic systems have great potential for the future of health care. However, current methods of DNA amplification and detection require bulky and expensive equipment, many steps, and long process times, which complicate their integration into POC devices. We have combined an isothermal DNA amplification method, recombinase polymerase amplification, with an electrochemical stem-loop (S-L) probe DNA detection technique. By combining these methods, we have created a system that is able to specifically amplify and detect as few as 10 copies/mu L Staphylococcus epidermidis DNA with a total time to result of 70-75 min.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Materialteknik -- Pappers-, massa- och fiberteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Materials Engineering -- Paper, Pulp and Fiber Technology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
ACS Omega
(Search for host publication in LIBRIS)
To the university's database