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Escherichia coli EC...
Escherichia coli EC93 deploys two plasmid- encoded class I contact- dependent growth inhibition systems for antagonistic bacterial interactions
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- Wäneskog, Marcus (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi,Institutionen för cell- och molekylärbiologi
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- Halvorsen, Tiffany (author)
- Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA.
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- Filek, Klara (author)
- Uppsala Univ, Dept Cell & Mol Biol, Uppsala, Sweden.;Univ Zagreb, Dept Biol, Zagreb, Croatia.
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- Xu, Feifei (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Molekylär evolution
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- Hammarlöf, Disa L. (author)
- Uppsala universitet,KTH,Science for Life Laboratory, SciLifeLab,Uppsala Univ, Dept Cell & Mol Biol, Uppsala, Sweden.;KTH, Sci Life Lab, Stockholm, Sweden.,Mikrobiologi och immunologi
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- Hayes, Christopher S. (author)
- Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA.
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- Braaten, Bruce A. (author)
- Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA.
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- Low, David A. (author)
- Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA.
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- Poole, Stephen J. (author)
- Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA.
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- Koskiniemi, Sanna, 1980- (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Mikrobiologi och immunologi
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(creator_code:org_t)
- MICROBIOLOGY SOC, 2021
- 2021
- English.
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In: Microbial Genomics. - : MICROBIOLOGY SOC. - 2057-5858. ; 7:3
- Related links:
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https://doi.org/10.1...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The phenomenon of contact- dependent growth inhibition (CDI) and the genes required for CDI (cdiBAI) were identified and isolated in 2005 from an Escherichia coli isolate (EC93) from rats. Although the cdiBAIEC93 locus has been the focus of extensive research during the past 15 years, little is known about the EC93 isolate from which it originates. Here we sequenced the EC93 genome and find two complete and functional cdiBAI loci (including the previously identified cdi locus), both carried on a large 127 kb plasmid. These cdiBAI systems are differentially expressed in laboratory media, enabling EC93 to outcompete E. coli cells lacking cognate cdiI immunity genes. The two CDI systems deliver distinct effector peptides that each dissipate the membrane potential of target cells, although the two toxins display different toxic potencies. Despite the differential expression and toxic potencies of these CDI systems, both yielded similar competitive advantages against E. coli cells lacking immunity. This can be explained by the fact that the less expressed cdiBAI system (cdiBAIEC93-2) delivers a more potent toxin than the highly expressed cdiBAIEC93-1 system. Moreover, our results indicate that unlike most sequenced CDI+ bacterial isolates, the two cdi loci of E. coli EC93 are located on a plasmid and are expressed in laboratory media.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- competition
- contact-dependent growth inhibition
- Escherichia coli
- genome
- regulation
- toxin
- toxic potency
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Wäneskog, Marcus
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Halvorsen, Tiffa ...
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Filek, Klara
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Xu, Feifei
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Hammarlöf, Disa ...
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Hayes, Christoph ...
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show more...
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Braaten, Bruce A ...
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Low, David A.
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Poole, Stephen J ...
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Koskiniemi, Sann ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Microbial Genomi ...
- By the university
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Royal Institute of Technology
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Uppsala University