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Sökning: onr:"swepub:oai:DiVA.org:kth-29841" > A single fixation p...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03351naa a2200469 4500 | |
| 001 | oai:DiVA.org:kth-29841 | |
| 003 | SwePub | |
| 008 | 110217s2010 | |||||||||||000 ||eng| | |
| 009 | oai:prod.swepub.kib.ki.se:120462390 | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-298412 URI |
| 024 | 7 | a https://doi.org/10.1016/j.jprot.2009.10.0122 DOI |
| 024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1204623902 URI |
| 040 | a (SwePub)kthd (SwePub)ki | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Stadler, Charlotteu KTH,Proteomik4 aut0 (Swepub:kth)u1r100ik |
| 245 | 1 0 | a A single fixation protocol for proteome-wide immunofluorescence localization studies |
| 264 | 1 | b Elsevier BV,c 2010 |
| 338 | a print2 rdacarrier | |
| 500 | a QC 20110223 3rd EuPA Congress, Stockholm, SWEDEN, JUN 14-17, 2009 | |
| 520 | a Immunofluorescence microscopy is a valuable tool for analyzing protein expression and localization at a subcellular level thus providing information regarding protein function, interaction partners and its role in cellular processes. When performing sample fixation, parameters such as difference in accessibility of proteins present in various cellular compartments as well as the chemical composition of the protein to be studied, needs to be taken into account. However, in systematic and proteome-wide efforts, a need exists for standard fixation protocol(s) that works well for the majority of all proteins independent of subcellular localization. Here, we report on a study with the goal to find a standardized protocol based on the analysis of 18 human proteins localized in 11 different organelles and subcellular structures. Six fixation protocols were tested based on either dehydration by alcohols (methanol, ethanol or iso-propanol) or cross-linking by paraformaldehyde followed by detergent permeabilization (Triton X-100 or saponin) in three human cell lines. Our results show that cross-linking is essential for proteome-wide localization studies and that cross-linking using paraformaldehyde followed by Triton X-100 permeabilization successfully can be used as a single fixation protocol for systematic studies. | |
| 650 | 7 | a TEKNIK OCH TEKNOLOGIERx Industriell bioteknik0 (SwePub)2092 hsv//swe |
| 650 | 7 | a ENGINEERING AND TECHNOLOGYx Industrial Biotechnology0 (SwePub)2092 hsv//eng |
| 653 | a Antibody | |
| 653 | a Confocal microscopy | |
| 653 | a Fixation | |
| 653 | a Immunofluorescence | |
| 653 | a Organelle | |
| 653 | a Permeabilization | |
| 653 | a Bioengineering | |
| 653 | a Bioteknik | |
| 700 | 1 | a Skogs, Marieu KTH,Proteomik4 aut0 (Swepub:kth)u1hik3sg |
| 700 | 1 | a Brismar, Hjalmaru Karolinska Institutet,KTH,Cellens fysik4 aut0 (Swepub:kth)u1232ew5 |
| 700 | 1 | a Uhlén, Mathiasu KTH,Proteomik4 aut0 (Swepub:kth)u1dulvmw |
| 700 | 1 | a Lundberg, Emmau KTH,Proteomik4 aut0 (Swepub:kth)u12bylj1 |
| 710 | 2 | a KTHb Proteomik4 org |
| 773 | 0 | t Journal of Proteomicsd : Elsevier BVg 73:6, s. 1067-1078q 73:6<1067-1078x 1874-3919x 1876-7737 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-29841 |
| 856 | 4 8 | u https://doi.org/10.1016/j.jprot.2009.10.012 |
| 856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:120462390 |
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