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Boosting the Electr...
Boosting the Electrochemical Performance of PI-5-CA/C-SWCNT Nanohybrid for Sensitive Detection of E. coli O157:H7 From the Real Sample
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- Wang, Huan (author)
- Huazhong Univ Life Sci & Technol, Coll Life Sci & Technol, Adv Biomat & Tissue Engn Ctr, Wuhan, Peoples R China.
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- Fan, Yanmiao (author)
- KTH,Ytbehandlingsteknik
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- Yang, Qiaoli (author)
- Huazhong Univ Life Sci & Technol, Coll Life Sci & Technol, Adv Biomat & Tissue Engn Ctr, Wuhan, Peoples R China.
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- Sun, Xiaoyu (author)
- Huazhong Univ Life Sci & Technol, Coll Life Sci & Technol, Adv Biomat & Tissue Engn Ctr, Wuhan, Peoples R China.
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- Liu, Hao (author)
- Huazhong Univ Life Sci & Technol, Coll Life Sci & Technol, Adv Biomat & Tissue Engn Ctr, Wuhan, Peoples R China.
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- Chen, Wei (author)
- Huazhong Univ Life Sci & Technol, Coll Life Sci & Technol, Adv Biomat & Tissue Engn Ctr, Wuhan, Peoples R China.
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- Aziz, Ayesha (author)
- Huazhong Univ Life Sci & Technol, Coll Life Sci & Technol, Adv Biomat & Tissue Engn Ctr, Wuhan, Peoples R China.
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- Wang, Shenqi (author)
- Huazhong Univ Life Sci & Technol, Coll Life Sci & Technol, Adv Biomat & Tissue Engn Ctr, Wuhan, Peoples R China.
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Huazhong Univ Life Sci & Technol, Coll Life Sci & Technol, Adv Biomat & Tissue Engn Ctr, Wuhan, Peoples R China Ytbehandlingsteknik (creator_code:org_t)
- 2022-02-10
- 2022
- English.
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In: Frontiers in Chemistry. - : Frontiers Media SA. - 2296-2646. ; 10
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Abstract
Subject headings
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- Redox activity is an important indicator for evaluating electrochemical biosensors. In this work, we have successfully polymerized indole-5-carboxylic acid into poly-5-carboxyindole nanomaterials (PI-5-CA), using its superior redox activity, and introduced carboxylated single-walled carbon nanotubes (C-SWCNTs) to synthesize a composite material. Finally, a synthesized composite material was used for the modification of the glass carbon electrode to fabricate the PI-5-CA/C-SWCNTs/GCE-based immunosensor and was successfully applied for the sensitive detection of E. coli O157:H7. The fabricated immunosensor exhibited an outstanding electrocatalytic activity toward the detection of E. coli O157:H7 with a remarkably lowest limit of detection (2.5 CFU/ml, LOD = 3 SD/k, n = 3) and has a wide linear range from 2.98x10(1) to 2.98x10(7) CFU/ml. Inspired from the excellent results, the fabricated electrode was applied for the detection of bacteria from real samples (water samples) with a good recovery rate (98.13-107.69%) as well as an excellent stability and specificity. Owing to its simple preparation, excellent performance, and detection time within 30 min, our proposed immunosensor will open a new horizon in different fields for the sensitive detection of bacteria from real samples.
Subject headings
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Livsmedelsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Food Science (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- poly-5-carboxyindole
- carboxylated single-walled carbon nanotubes
- E. coli O157:H7
- electrochemical immunosensor
- indole-5-carboxylic acid
- plate counting method
Publication and Content Type
- ref (subject category)
- art (subject category)
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