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A Uniform and Isotr...
A Uniform and Isotropic Cytoskeletal Tiling Fills Dendritic Spines
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Eberhardt, F. (author)
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Bushong, E. A. (author)
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Phan, S. (author)
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Peltier, S. (author)
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Monteagudo-Mesas, P. (author)
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- Weinkauf, Tino, 1974- (author)
- KTH,Beräkningsvetenskap och beräkningsteknik (CST)
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Herz, A. V. M. (author)
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Stemmler, M. (author)
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Ellisman, M. (author)
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(creator_code:org_t)
- Society for Neuroscience, 2022
- 2022
- English.
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In: eNeuro. - : Society for Neuroscience. - 2373-2822. ; 9:5
- Related links:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Dendritic spines are submicron, subcellular compartments whose shape is defined by actin filaments and associated proteins. Accurately mapping the cytoskeleton is a challenge, given the small size of its components. It remains unclear whether the actin-associated structures analyzed in dendritic spines of neurons in vitro apply to dendritic spines of intact, mature neurons in situ. Here, we combined advanced preparative methods with multitilt serial section electron microscopy (EM) tomography and computational analysis to reveal the full three-dimensional (3D) internal architecture of spines in the intact brains of male mice at nanometer resolution. We compared hippocampal (CA1) pyramidal cells and cerebellar Purkinje cells in terms of the length distribution and connectivity of filaments, their branching-angles and absolute orientations, and the elementary loops formed by the network. Despite differences in shape and size across spines and between spine heads and necks, the internal organization was remarkably similar in both neuron types and largely homogeneous throughout the spine volume. In the tortuous mesh of highly branched and interconnected filaments, branches exhibited no preferred orientation except in the immediate vicinity of the cell membrane. We found that new filaments preferentially split off from the convex side of a bending filament, consistent with the behavior of Arp2/3-mediated branching of actin under mechanical deformation. Based on the quantitative analysis, the spine cytoskeleton is likely subject to considerable mechanical force in situ.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- actin cytoskeleton
- cerebellar Purkinje cell
- dendritic spines in situ
- EM tomography
- hippocampal pyramidal cell
- image segmentation
- Actins
- Animals
- Cytoskeleton
- Dendritic Spines
- Hippocampus
- Male
- Mice
- Neurons
- ketamine
- xylazine
- actin
- actin filament
- animal cell
- animal experiment
- animal model
- animal tissue
- Article
- brain tissue
- C57BL/6N mouse
- cell membrane
- controlled study
- dendritic spine
- electron microscopy
- electron tomography
- endoplasmic reticulum
- image reconstruction
- machine learning
- molecular dynamics
- morphometry
- mouse
- nonhuman
- postsynaptic density
- Purkinje cell
- pyramidal nerve cell
- quantitative analysis
- synapse
- three dimensional echography
- animal
- metabolism
- nerve cell
Publication and Content Type
- ref (subject category)
- art (subject category)
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