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High cardiomyocyte ...
High cardiomyocyte diversity in human early prenatal heart development
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- Sylven, Christer (author)
- Karolinska Inst, Dept Med, Huddinge, Sweden.
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- Wardell, Eva (author)
- Karolinska Institutet
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- Månsson-Broberg, Agneta (author)
- Karolinska Institutet
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- Cingolani, Eugenio (author)
- Smidt Heart Inst, Los Angeles, CA USA.
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- Ampatzis, Konstantinos (author)
- Karolinska Institutet
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- Larsson, Ludvig (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab,KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden.
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- Björklund, Åsa K. (author)
- Uppsala universitet,Molekylär evolution,Science for Life Laboratory, SciLifeLab
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- Giacomello, Stefania (author)
- KTH,Science for Life Laboratory, SciLifeLab,Genteknologi,KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden.
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Karolinska Institutet Karolinska Inst, Dept Med, Huddinge, Sweden (creator_code:org_t)
- Elsevier BV, 2023
- 2023
- English.
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In: ISCIENCE. - : Elsevier BV. - 2589-0042. ; 26:1, s. 105857-
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Abstract
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- Cardiomyocytes play key roles during cardiogenesis, but have poorly understood features, especially in prenatal stages. Here, we characterized human prenatal cardiomyocytes, 6.5-7 weeks post-conception, by integrating single-cell RNA sequencing, spatial transcriptomics, and ligand-receptor interaction information. Using a computational workflow developed to dissect cell type heterogeneity, localize cell types, and explore their molecular interactions, we identified eight types of developing cardiomyocyte, more than double compared to the ones identified in the Human Developmental Cell Atlas. These have high variability in cell cycle activity, mitochondrial content, and connexin gene expression, and are differentially distributed in the ventricles, including outflow tract, and atria, including sinoatrial node. Moreover, cardiomyocyte ligand-receptor crosstalk is mainly with non-cardiomyocyte cell types, encompassing cardiogenesis-related pathways. Thus, early prenatal human cardiomyocytes are highly heterogeneous and develop unique location-dependent properties, with complex ligand-receptor crosstalk. Further elucidation of their developmental dynamics may give rise to new therapies.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kardiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cardiac and Cardiovascular Systems (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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