A beta seeds resist inactivation by formaldehyde

Fritschi, Sarah K. (author): German Centre Neurodegenerat Disease DZNE, Germany; University of Tubingen, Germany; University of Tubingen, Germany

Ye, Lan (author): German Centre Neurodegenerat Disease DZNE, Germany; University of Tubingen, Germany; University of Tubingen, Germany

Mahler, Jasmin (author): German Centre Neurodegenerat Disease DZNE, Germany; University of Tubingen, Germany; University of Tubingen, Germany

Buehler, Anika (author): German Centre Neurodegenerat Disease DZNE, Germany; University of Tubingen, Germany

Baumann, Frank (author): German Centre Neurodegenerat Disease DZNE, Germany; University of Tubingen, Germany

Neumann, Manuela (author): German Centre Neurodegenerat Disease DZNE, Germany; University of Tubingen, Germany

Walker, Lary C. (author): Emory University, GA 30329 USA; Emory University, GA 30322 USA

Jucker, Mathias (author): German Centre Neurodegenerat Disease DZNE, Germany; University of Tubingen, Germany

(creator_code:org_t)

2014-09-06
2014
English.
In: Acta Neuropathologica. - : Springer Verlag (Germany). - 0001-6322 .- 1432-0533. ; 128:4, s. 477-484

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Abstract Subject headings

Cerebral beta-amyloidosis can be exogenously induced by the intracerebral injection of brain extracts containing aggregated beta-amyloid (A beta) into young, pre-depositing A beta precursor protein- (APP) transgenic mice. Previous work has shown that the induction involves a prion-like seeding mechanism in which the seeding agent is aggregated A beta itself. Here we report that the beta-amyloid-inducing activity of Alzheimers disease (AD) brain tissue or aged APP-transgenic mouse brain tissue is preserved, albeit with reduced efficacy, after formaldehyde fixation. Moreover, spectral analysis with amyloid conformation-sensitive luminescent conjugated oligothiophene dyes reveals that the strain-like properties of aggregated A beta are maintained in fixed tissues. The resistance of A beta seeds to inactivation and structural modification by formaldehyde underscores their remarkable durability, which in turn may contribute to their persistence and spread within the body. The present findings can be exploited to establish the relationship between the molecular structure of A beta aggregates and the variable clinical features and disease progression of AD even in archived, formalin-fixed autopsy material.

By the author/editor: Fritschi, Sarah ...; Cintron, Amarall ...; Ye, Lan; Mahler, Jasmin; Buehler, Anika; Baumann, Frank; show more...; Neumann, Manuela; Nilsson, Peter; Hammarström, Per; Walker, Lary C.; Jucker, Mathias; show less...

About the subject

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