NMR studies of erythrocytes immobilized in agarose and alginate gels

Lundberg, Peter (author): Linköpings universitet,Avdelningen för radiologiska vetenskaper,Hälsouniversitetet,Centrum för medicinsk bildvetenskap och visualisering, CMIV,Region Östergötland, Radiofysikavdelningen US,Department of Biochemistry, University of Sydney, Sydney, New South Wales. 2006, Australia

Berners-Price, Susan J. (author): Division of Science and Technology, Griffith University, Nathan, Queensland, 4111, Australia

Roy, Sushmita (author): Department of Biochemistry, University of Sydney, Sydney, New South Wales. 2006, Australia

Kuchel, Philip W. (author): Department of Biochemistry, University of Sydney, Sydney, New South Wales. 2006, Australia

(creator_code:org_t)

John Wiley & Sons, 1992
1992
English.
In: Magnetic Resonance in Medicine. - : John Wiley & Sons. - 0740-3194 .- 1522-2594. ; 25:2, s. 273-288

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; show less...

Abstract Subject headings

31P and 13C NMR were used to study the energy metabolism in perfused, human erythrocytes. The erythrocytes were immobilized in agarose threads, Ca- or Ba-alginate beads, and Ba-alginate-coated agarose threads. Erythrocytes were easily washed out from the agarose threads, but not from alginate-containing gels. Various small molecules, such as hypophosphite, dimethyl methylphosphonate, and methylphosphonate, were taken up from the perfusion medium in a normal manner. In addition, the 2,3-bisphosphoglycerate (2,3-DPG) chemical shifts were sensitive to the oxygen partial pressure suggesting that O2 molecules were diffusing through the gel and modifying the binding of 2,3-DPG to hemoglobin. A combination of inosine and pyruvate stimulated the synthesis of 2,3-DPG, but only if inorganic phosphate was present in the perfusion medium. Inosine only resulted in a dramatic rise in the intracellular sugarphosphate concentrations. Furthermore, [2-13C]glucose was converted to [2-13C]lactate by immobilized cells at a rate which was comparable to that in a control suspension. In summary, immobilization in Ba-alginate-coated agarose threads was an efficient way of trapping human erythrocytes for whole cell NMR investigations.

By the author/editor: Lundberg, Peter; Berners-Price, S ...; Roy, Sushmita; Kuchel, Philip W ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Physical Chemist ...

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