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Redox cycling induc...
Redox cycling induces spermptosis and necrosis in stallion spermatozoa while the hydroxyl radical (OH center dot) only induces spermptosis
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- Munoz, P. Martin (författare)
- University of Extremadura, Spain
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- Anel-Lopez, L. (författare)
- University of Leon, Spain
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- Ortiz-Rodriguez, J. M. (författare)
- University of Extremadura, Spain
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- Alvarez, M. (författare)
- University of Leon, Spain
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- de Paz, P. (författare)
- University of Leon, Spain
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- Balao da Silva, C. (författare)
- Super Agriculture School Elvas, Portugal
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- Rodriguez-Martinez, Heriberto (författare)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten
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- Gil, M. C. (författare)
- University of Extremadura, Spain
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- Anel, L. (författare)
- University of Leon, Spain
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- Pena, F. J. (författare)
- University of Extremadura, Spain
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- Ortega Ferrusola, C. (författare)
- University of Leon, Spain
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(creator_code:org_t)
- 2017-08-17
- 2018
- Engelska.
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Ingår i: Reproduction in domestic animals. - : WILEY. - 0936-6768 .- 1439-0531. ; 53:1, s. 54-67
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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http://liu.diva-port...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Oxidative stress is a major factor explaining sperm dysfunction of spermatozoa surviving freezing and thawing and is also considered a major inducer of a special form of apoptosis, visible after thawing, in cryopreserved spermatozoa. To obtain further insights into the link between oxidative stress and the induction of apoptotic changes, stallion spermatozoa were induced to oxidative stress through redox cycling after exposure to 2-methyl-1,4-naphthoquinone (menadione), or hydroxyl radical formation after FeSO4 exposure. Either exposure induced significant increases (p amp;lt; 0.05) in two markers of lipid peroxidation: 8-iso-PGF(2) and 4-hydroxynonenal (4-HNE). While both treatments induced changes indicative of spermptosis (caspase-3 activation and decreased mitochondrial membrane potential) (p amp;lt; 0.01), menadione induced sperm necrosis and a dramatic reduction in motility and thiol content in stallion spermatozoa. Thus, we provided evidence that oxidative stress underlies spermptosis, and thiol content is a key factor for stallion sperm function.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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