SwePub
Sök i LIBRIS databas

  Utökad sökning

id:"swepub:oai:DiVA.org:liu-145125"
 

Sökning: id:"swepub:oai:DiVA.org:liu-145125" > Seminal plasma anti...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004809naa a2200409 4500
001oai:DiVA.org:liu-145125
003SwePub
008180219s2018 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-1451252 URI
0247 a https://doi.org/10.1016/j.theriogenology.2017.10.0352 DOI
040 a (SwePub)liu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Li, Junweiu Univ Murcia, Spain4 aut
2451 0a Seminal plasma antioxidants are directly involved in boar sperm cryotolerance
264 1b ELSEVIER SCIENCE INC,c 2018
338 a electronic2 rdacarrier
500 a Funding Agencies|Seneca Foundation Murcia of Spain [19892/GERM-15]; China Scholarship Council [2013/3009]
520 a Boar ejaculates are ejected in fractions with a specific composition in terms of sperm numbers and seminal plasma (SP), which is reflected in the varying sperm cryotolerance observed among different fractions. As boar sperm are particularly sensitive to oxidative stress, this study evaluated the role of SP antioxidants in the observed differences in sperm cryotolerance among ejaculate fractions. Ten ejaculates from five boars were manually collected in fractions: the first 10 mL of the sperm-rich fraction (SRF), the rest of the SRF and the post-SRF. Semen samples comprising the entire ejaculate (EE) were created by proportionally mixing the three fractions described above. Each of the 40 resulting semen samples was split into two aliquots: one was used for sperm cryopreservation following a standard protocol utilizing 0.5-mL straws, and the other was used to collect SP for antioxidant assessment. Frozen-thawed (FT) sperm from the SRF (the first 10 mL of the SRF and the rest of the SRF) and those from post-SRF were of the highest and worst quality, respectively, which was measured in terms of total and objective progressive motility and viability (P amp;lt; 0.01). Viable FT sperm from the post-SRF generated more reactive oxygen species and experienced more lipid peroxidation than those from the SRF (both the first 10 mL and the rest of the SRF) (P amp;lt; 0.01). The percentage of FT sperm exhibiting fragmented nuclear DNA did not differ among ejaculate fractions and the EE. Catalase, glutathione peroxidase and glutathione peroxidase 5 (GPx-5) were lowest in SP from the first 10 mL of the SRF (P amp;lt; 0.001), whereas superoxide dismutase (SOD) and paraoxonase 1 (PON-1) were highest in SP of the SRF (both the first 10 mL and the rest of the SRF) (P amp;lt; 0.01). Trolox-equivalent antioxidant capacity (TEAC) and the ferric-reducing ability of plasma (FRAP) were highest in SP from the first 10 mL of the SRF and lowest in the post-SRF (P amp;lt; 0.001), whereas cupric-reducing antioxidant capacity was lowest (P amp;lt; 0.05) in SP from the first 10 mL of the SRF. Regression analyses indicated that certain SP antioxidants had good predictive value for post-thaw recovery rates of total motility (R-2 = 54.8%, P amp;lt; 0.001; including SOD, TEAC and FRAP) and viability (R-2 = 56.1%, P amp;lt; 0.001; including SOD, PON-1, GPx-5 and TEAC). These results demonstrated that certain SP antioxidants are positively involved in boar sperm cryotolerance, minimizing the oxidative stress imposed by cryogenic handling. (C) 2017 Elsevier Inc. All rights reserved.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Reproduktionsmedicin och gynekologi0 (SwePub)302202 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Obstetrics, Gynaecology and Reproductive Medicine0 (SwePub)302202 hsv//eng
653 a Boar; Ejaculate fractions; Sperm cryotolerance; Seminal plasma; Antioxidants
7001 a Barranco, Isabelu Univ Murcia, Spain4 aut
7001 a Tvarijonaviciute, Astau Univ Murcia, Spain4 aut
7001 a Molina, Manuel F.u Univ Murcia, Spain4 aut
7001 a Martinez, Emilio A.u Univ Murcia, Spain4 aut
7001 a Rodriguez-Martinez, Heribertou Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten4 aut0 (Swepub:liu)herro55
7001 a Parrilla, Inmaculadau Univ Murcia, Spain4 aut
7001 a Roca, Jordiu Univ Murcia, Spain4 aut
7102 a Univ Murcia, Spainb Avdelningen för barns och kvinnors hälsa4 org
7730 t Theriogenologyd : ELSEVIER SCIENCE INCg 107, s. 27-35q 107<27-35x 0093-691Xx 1879-3231
8564 u https://liu.diva-portal.org/smash/get/diva2:1183667/FULLTEXT01.pdfx primaryx Raw objecty fulltext:postprint
8564 u http://liu.diva-portal.org/smash/get/diva2:1183667/FULLTEXT01
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-145125
8564 8u https://doi.org/10.1016/j.theriogenology.2017.10.035

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy