Seminal Plasma Modifies the Transcriptional Pattern of the Endometrium and Advances Embryo Development in Pigs

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Seminal Plasma Modifies the Transcriptional Pattern of the Endometrium and Advances Embryo Development in Pigs

Martinez-Serrano, Cristina (author): Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain

Cambra, Josep M. (author): Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain

Parrilla, Inmaculada (author): Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain

Roca, Jordi (author): Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain

Ferreira-Dias, Graca (author): Univ Lisbon, Portugal

Pallares, Francisco J. (author): Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain

Lucas, Xiomara (author): Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain

Vazquez, Juan M. (author): Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain

Martinez, Emilio A. (author): Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain

Gil, Maria A. (author): Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain

Rodriguez-Martinez, Heriberto (author): Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten

Cuello, Cristina (author): Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain

Alvarez-Rodriguez, Manuel (author): Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten

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2019-12-18
2019
English.
In: Frontiers in Veterinary Science. - : Frontiers Media S.A.. - 2297-1769. ; 6

Related links:: https://liu.diva-por... (primary) (Raw object); show more...; https://doi.org/10.3...; https://urn.kb.se/re...; https://doi.org/10.3...; show less...

Journal article (peer-reviewed)

Abstract Subject headings

Background: Boar seminal plasma is rich in cytokines, which could influence the capability of spermatozoa to tolerate preservation.Objectives: To evaluate the involvement of boar seminal plasma cytokines in the changes experienced by boar spermatozoa during their storage, either in liquid or frozen state.Materials and Methods: In two separated experiments, semen samples from healthy and fertile boars were split in two aliquots, one centrifuged twice (1,500 ×g for 10 min) to harvest seminal plasma, whereas the other was either commercially extended (3 × 107 sperm/mL) and liquid-stored at 17°C during 144 h (n = 28, Experiment 1) or frozen-thawed using a standard 0.5 mL protocol (n = 27, Experiment 2). Sixteen cytokines were quantified using Luminex xMAP®. Sperm attributes (CASA-evaluated total and progressive motility; flow cytometry-evaluated sperm viability, production of intracellular H2O2 and O2•-" role="presentation" style="box-sizing: border-box; display: inline; line-height: normal; word-spacing: normal; overflow-wrap: normal; white-space: nowrap; float: none; direction: ltr; max-width: none; max-height: none; min-width: 0px; min-height: 0px; border: 0px; padding: 0px; margin: 0px; position: relative; outline: 0px !important;">O∙−2O2•- and levels of lipid peroxidation in viable spermatozoa) were evaluated either at 0, 72, or 144 h of liquid storage (Experiment 1) or before freezing and at 30- and 150-min post-thawing (Experiment 2).Results: Multiple linear regression models, with Bayesian approach for variable selection, revealed that the anti-inflammatory TGF-β2, TGF-β3, IL-1Ra, and IL-4 and the pro-inflammatory IL-8 and IL-18, predicted changes in sperm motility for liquid-stored semen while the anti-inflammatory IFN-γ was included in the models predicting changes in all sperm attributes for cryopreserved semen.Conclusion: Specific boar seminal plasma cytokines would contribute to modulate the structural and metabolic changes shown by spermatozoa during preservation, either in liquid or frozen state.

Subject headings

NATURVETENSKAP -- Biologi -- Utvecklingsbiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Developmental Biology (hsv//eng)

Keyword

seminal plasma; endometrium; transcriptome; embryo; preimplantation; pig

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By the author/editor: Martinez-Serrano ...; Cambra, Josep M.; Parrilla, Inmacu ...; Roca, Jordi; Ferreira-Dias, G ...; Pallares, Franci ...; show more...; Lucas, Xiomara; Vazquez, Juan M.; Martinez, Emilio ...; Gil, Maria A.; Rodriguez-Martin ...; Cuello, Cristina; Alvarez-Rodrigue ...; show less...

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NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Developmental Bi ...

Articles in the publication: Frontiers in Vet ...

By the university: Linköping University

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