Sökning: id:"swepub:oai:DiVA.org:liu-177416" >
SARS-CoV-2 rapid an...
SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious virus
-
- Nordgren, Johan (författare)
- Linköpings universitet,Avdelningen för molekylär medicin och virologi,Medicinska fakulteten
-
- Sharma, Sumit (författare)
- Linköpings universitet,Avdelningen för molekylär medicin och virologi,Medicinska fakulteten
-
- Olsson, Henrik (författare)
- Noviral Sweden AB, Sweden
-
visa fler...
-
- Jamtberg, Mikael (författare)
- Noviral Sweden AB, Sweden
-
- Falkeborn, Tina (författare)
- Linköpings universitet,Avdelningen för molekylär medicin och virologi,Medicinska fakulteten,Region Östergötland, Klinisk mikrobiologi
-
- Svensson, Lennart (författare)
- Karolinska Institutet,Linköpings universitet,Avdelningen för molekylär medicin och virologi,Medicinska fakulteten,Karolinska Inst, Sweden
-
- Hagbom, Marie (författare)
- Linköpings universitet,Avdelningen för molekylär medicin och virologi,Medicinska fakulteten
-
visa färre...
-
(creator_code:org_t)
- ELSEVIER, 2021
- 2021
- Engelska.
-
Ingår i: Journal of Clinical Virology. - : ELSEVIER. - 1386-6532 .- 1873-5967. ; 140
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background: The COVID-19 pandemic has highlighted the need for rapid, cost effective and easy-to-use diagnostic tools for SARS-CoV-2 infections that can be used in point of care settings to limit disease transmission. Objective: We evaluated two rapid antigen immunochromatographic tests, Abbott PanbioTM COVID-19 Ag Rapid Test (Panbio) and Zhejiang Orient Gene/Healgen Biotech Coronavirus Ag rapid test cassette (Orient gene) for detection of infectious SARS-CoV-2. Results: The tests were evaluated on nasopharyngeal samples taken from individuals having respiratory and/or COVID-19 related symptoms, which had been analyzed for SARS-CoV-2 RNA using real-time PCR. In total 156 PCR-positive, and 130 (Panbio) and 176 (Orient Gene) PCR-negative samples were analyzed. Overall sensitivity and specificity were 71.8% and 100% for Panbio and 79.5% and 74.4% for the Orient Gene test respectively. The false positives by the Orient Gene test were verified as SARS-CoV-2 negative by in-house real-time PCR assay and were negative for the four seasonal coronaviruses. Subgroup analysis revealed that the antigen tests had high sensitivity for samples with Ct-values <25 (>88%) and for samples containing infectious viruses as determined by cultivation on Vero cells, 94.1% and 97.1% for the Panbio and Orient gene tests, respectively. Furthermore, both tests had a sensitivity of <50 picogram for nucleocapsid protein. No sample with a Ct-value >27 was shown to contain infectious virus. Conclusion: The results indicate that the rapid antigen tests, especially the Panbio tests may be a valuable tool to detect contagious persons during the ongoing pandemic.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- SARS-CoV-2; Rapid antigen test; Infectious virus
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas