Search: id:"swepub:oai:DiVA.org:liu-186812" >
Epigenetic rewiring...
Epigenetic rewiring of pathways related to odour perception in immune cells exposed to SARS-CoV-2 in vivo and in vitro
-
- Huoman, Johanna (author)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Univ Hosp Bonn, Germany
-
- Sayyab, Shumaila (author)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten
-
- Apostolou, Eirini (author)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten
-
show more...
-
- Karlsson, Lovisa (author)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten
-
- Porcile, Lucas (author)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten
-
- Rizwan, Muhammad (author)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten
-
- Sharma, Sumit (author)
- Linköpings universitet,Avdelningen för molekylär medicin och virologi,Medicinska fakulteten
-
- Das, Jyotirmoy (author)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten
-
- Rosén, Anders (author)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten
-
- Lerm, Maria (author)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten
-
show less...
-
(creator_code:org_t)
- 2022-06-26
- 2022
- English.
-
In: Epigenetics. - New York, NY, United States : Taylor & Francis. - 1559-2294 .- 1559-2308. ; 17:13, s. 1875-1891
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://liu.diva-por... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- A majority of SARS-CoV-2 recoverees develop only mild-to-moderate symptoms, while some remain completely asymptomatic. Although viruses, including SARS-CoV-2, may evade host immune responses by epigenetic mechanisms including DNA methylation, little is known about whether these modifications are important in defence against and healthy recovery from COVID-19 in the host. To this end, epigenome-wide DNA methylation patterns from COVID-19 convalescents were compared to uninfected controls from before and after the pandemic. Peripheral blood mononuclear cell (PBMC) DNA was extracted from uninfected controls, COVID-19 convalescents, and symptom-free individuals with SARS-CoV-2-specific T cell-responses, as well as from PBMCs stimulated in vitro with SARS-CoV-2. Subsequently, the Illumina MethylationEPIC 850K array was performed, and statistical/bioinformatic analyses comprised differential DNA methylation, pathway over-representation, and module identification analyses. Differential DNA methylation patterns distinguished COVID-19 convalescents from uninfected controls, with similar results in an experimental SARS-CoV-2 infection model. A SARS-CoV-2-induced module was identified in vivo, comprising 66 genes of which six (TP53, INS, HSPA4, SP1, ESR1, and FAS) were present in corresponding in vitro analyses. Over-representation analyses revealed involvement in Wnt, muscarinic acetylcholine receptor signalling, and gonadotropin-releasing hormone receptor pathways. Furthermore, numerous differentially methylated and network genes from both settings interacted with the SARS-CoV-2 interactome. Altered DNA methylation patterns of COVID-19 convalescents suggest recovery from mild-to-moderate SARS-CoV-2 infection leaves longstanding epigenetic traces. Both in vitro and in vivo exposure caused epigenetic modulation of pathways thataffect odour perception. Future studies should determine whether this reflects host-induced protective antiviral defense or targeted viral hijacking to evade host defence.
Subject headings
- LANTBRUKSVETENSKAPER -- Veterinärmedicin -- Medicinsk biovetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Veterinary Science -- Medical Bioscience (hsv//eng)
Keyword
- SARS-CoV-2; mild-to-moderate; PBMC; DNA methylation; in vitro stimulation; module identification; network analysis; interactome
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Huoman, Johanna
-
Sayyab, Shumaila
-
Apostolou, Eirin ...
-
Karlsson, Lovisa
-
Porcile, Lucas
-
Rizwan, Muhammad
-
show more...
-
Sharma, Sumit
-
Das, Jyotirmoy
-
Rosén, Anders
-
Lerm, Maria
-
show less...
- About the subject
-
- AGRICULTURAL SCIENCES
-
AGRICULTURAL SCI ...
-
and Veterinary Scien ...
-
and Medical Bioscien ...
- Articles in the publication
-
Epigenetics
- By the university
-
Linköping University