SwePub
Sök i LIBRIS databas

  Extended search

id:"swepub:oai:DiVA.org:liu-24725"
 

Search: id:"swepub:oai:DiVA.org:liu-24725" > Biglycan gene expre...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Biglycan gene expression in UVR-exposed rabbit corneas

Podskochy, Alexander, 1971- (author)
Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Oftalmologi,Ögonkliniken US
Koulikovska, Marina, 1970- (author)
Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Oftalmologi,Ögonkliniken US
Fagerholm, Per, 1948- (author)
Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Oftalmologi,Ögonkliniken US
show more...
van der Ploeg, Ingeborg (author)
Karolinska Institutet
show less...
 (creator_code:org_t)
2004-03-23
2004
English.
In: Acta Ophthalmologica Scandinavica. - : Wiley. - 1395-3907 .- 1600-0420. ; 82:2, s. 200-204
  • Journal article (peer-reviewed)
Abstract Subject headings
Close  
  • Purpose: It is known that stromal proteoglycans play an important role in the hydration and transparency of the mammalian cornea. Proteoglycans have been described as a pathological deposit in climatic proteoglycan stromal keratopathy, which is associated with chronic ultraviolet radiation (UVR) exposure. The expression of dermatan sulfate proteoglycan biglycan in the cornea was thus studied after exposure of rabbit eyes to UVR. Methods: New Zealand albino rabbit corneas were exposed to UVR at 310 nm at the dose producing biomicroscopically significant keratitis (0.47 J/cm2 ). Animals were killed 3, 7 and 28 days after exposure (five rabbits in each group). Five rabbits were used as controls and did not receive any UVR treatment. Expression of biglycan mRNA in the corneas was investigated by competitive reverse transcription-polymerase chain reaction (RT-PCR). Results: There was no expression of biglycan mRNA in the control group. In the UVR-exposed groups, biglycan mRNA had still not been expressed 3 days after exposure. The expression of biglycan mRNA was observed in all UVR-treated corneas 7 days after exposure (p < 0.05). By 28 days after UVR exposure the expression of biglycan mRNA had decreased (not statistically significant). Conclusions: There is no detectable biglycan gene expression in the normal rabbit cornea. Ultraviolet radiation exposure leads to a distinct expression of biglycan mRNA in the rabbit cornea that decreases 4 weeks after exposure, indicating the involvement of biglycan in the corneal repair process. Biglycan appears to be a novel marker of corneal wound healing.

Keyword

MEDICINE
MEDICIN

Publication and Content Type

ref (subject category)
art (subject category)

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Search outside SwePub

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view