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Increased expressio...
Increased expression of IRF4 and ETS1 in CD4+ cells from patients with intermittent allergic rhinitis
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- Bruhn, Sören (author)
- Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet,Genepathway, Inc., San Diego, CA, USA
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- Barrenäs, Fredrik (author)
- Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet
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- Mobini, Reza, 1965 (author)
- Östergötlands Läns Landsting
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- Andersson, Bengt A., 1952 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology,Sahlgrenska University Hospital
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- Chavali, Sreenivas (author)
- MRC Lab Molecular Biol, Cambridge, UK
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- Egan, B. S. (author)
- Genepathway, Inc., San Diego, CA, USA
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- Hovig, E. (author)
- Norwegian Radium Hospital
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- Sandve, G. K. (author)
- University of Oslo
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- Langston, M. A. (author)
- University of Tennessee, Knoxville, TN, USA
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- Rogers, G. (author)
- University of Tennessee, Knoxville, TN, USA
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- Wang, Hui (author)
- Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet
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- Benson, Mikael (author)
- Linköpings universitet,Hälsouniversitetet,Pediatrik
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(creator_code:org_t)
- 2011-09-15
- 2012
- English.
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In: Allergy. European Journal of Allergy and Clinical Immunology. - : John Wiley and Sons. - 0105-4538 .- 1398-9995. ; 67:1, s. 33-40
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Abstract
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- Background: The transcription factor (TF) IRF4 is involved in the regulation of Th1, Th2, Th9, and Th17 cells, and animal studies have indicated an important role in allergy. However, IRF4 and its target genes have not been examined in human allergy. Methods: IRF4 and its target genes were examined in allergen-challenged CD4+ cells from patients with IAR, using combined gene expression microarrays and chromatin immunoprecipitation chips (ChIP-chips), computational target prediction, and RNAi knockdowns. Results: IRF4 increased in allergen-challenged CD4+ cells from patients with IAR, and functional studies supported its role in Th2 cell activation. IRF4 ChIP-chip showed that IRF4 regulated a large number of genes relevant to Th cell differentiation. However, neither Th1 nor Th2 cytokines were the direct targets of IRF4. To examine whether IRF4 induced Th2 cytokines via one or more downstream TFs, we combined gene expression microarrays, ChIP-chips, and computational target prediction and found a putative intermediary TF, namely ETS1 in allergen-challenged CD4+ cells from allergic patients. ETS1 increased significantly in allergen-challenged CD4+ cells from patients compared to controls. Gene expression microarrays before and after ETS1 RNAi knockdown showed that ETS1 induced Th2 cytokines as well as disease-related pathways. Conclusions: Increased expression of IRF4 in allergen-challenged CD4+ cells from patients with intermittent allergic rhinitis leads to activation of a complex transcriptional program, including Th2 cytokines.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Pediatrik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Pediatrics (hsv//eng)
Keyword
- Allergy
- transcription factor
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Bruhn, Sören
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Barrenäs, Fredri ...
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Mobini, Reza, 19 ...
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Andersson, Bengt ...
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Chavali, Sreeniv ...
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Egan, B. S.
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Hovig, E.
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Sandve, G. K.
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Langston, M. A.
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Rogers, G.
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Wang, Hui
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Benson, Mikael
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Pediatrics
- Articles in the publication
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Allergy. Europea ...
- By the university
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Linköping University
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University of Gothenburg