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Sökning: id:"swepub:oai:DiVA.org:liu-89513" > Osteogenically-indu...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004008naa a2200421 4500
001oai:DiVA.org:liu-89513
003SwePub
008130226s2013 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-895132 URI
0247 a https://doi.org/10.3109/2000656X.2012.7314112 DOI
040 a (SwePub)liu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Sommar, Pehru Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet4 aut0 (Swepub:liu)pehla40
2451 0a Osteogenically-induced human dermal fibroblasts as a tool to regenerate bone
264 1b Informa Healthcare,c 2013
338 a print2 rdacarrier
520 a Engineering of bone tissue could help to overcome the need for extensive reconstruction and associated donor site morbidity, and it has been proposed that osteogenic biomaterials, which are scaffolds that contain osteocompetent cells, could be used to fill large bone defects. This study investigated the potential of osteogenically-induced human dermal fibroblasts cultured on gelatin microcarriers combined with platelet-rich plasma in a model of a femoral defect in athymic rats. Defects were transplanted with one of the following six combinations: 1 = sodium chloride, 2 = platelet-rich plasma, 3 = microcarriers + platelet-rich plasma, 4 = human dermal fibroblasts on microcarriers + platelet-rich plasma, 5 = human osteoblasts on microcarriers + platelet-rich plasma, and 6 = osteogenically induced human dermal fibroblasts on microcarriers + platelet-rich plasma. The femoral defects were assessed 4 weeks postoperatively with computed tomography (CT), routine histological staining, fluorescence in situ hybridisation, and polyclonal antibodies directed towards osteocalcin and osteonectin. Radiographs of all groups taken 4 weeks postoperatively showed unhealed defects. Femoral defects transplanted with osteogenically-induced human dermal fibroblasts on microcarriers (group 6) contained dense clusters of cells with large quantities of extracellular matrix. These clusters were exclusive to this group and stained strongly for osteocalcin and osteonectin. Fluorescence in situ hybridisation showed viable human cells in femoral defects that had been transplanted with microcarriers seeded with cells, which confirmed the survival of implanted cells. In conclusion, osteogenically-induced human dermal fibroblasts survived in this new niche, and bone-like structures were apparent in the defects.
653 a Tissue engineering
653 a stem cells
653 a human dermal fibroblasts
653 a differentiation
653 a osteogenic induction
653 a formation of bone
653 a MEDICINE
653 a MEDICIN
7001 a Junker, Johanu Linköpings universitet,Cellbiologi,Hälsouniversitetet4 aut0 (Swepub:liu)johju92
7001 a Strandenes, Eivindu Haukeland Hospital, Norway4 aut
7001 a Ness, Charlotteu Haukeland Hospital, Norway4 aut
7001 a Hansson, Thomasu Östergötlands Läns Landsting,Linköpings universitet,Kirurgi,Hälsouniversitetet,Hand- och plastikkirurgiska kliniken US4 aut0 (Swepub:liu)thoha80
7001 a Johnson, Hansu Östergötlands Läns Landsting,Linköpings universitet,Kirurgi,Hälsouniversitetet,Hand- och plastikkirurgiska kliniken US4 aut0 (Swepub:liu)hanjo30
7001 a Kratz, Gunnaru Östergötlands Läns Landsting,Linköpings universitet,Hand och plastikkirurgi,Hälsouniversitetet,Hand- och plastikkirurgiska kliniken US4 aut0 (Swepub:liu)gunkr37
7102 a Linköpings universitetb Institutionen för klinisk och experimentell medicin4 org
7730 t Journal of Plastic Surgery and Hand Surgeryd : Informa Healthcareg 47:1, s. 8-13q 47:1<8-13x 2000-656Xx 2000-6764
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-89513
8564 8u https://doi.org/10.3109/2000656X.2012.731411

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