id:"swepub:oai:DiVA.org:liu-90744"
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04201naa a2200421 4500 | |
| 001 | oai:DiVA.org:liu-90744 | |
| 003 | SwePub | |
| 008 | 130405s2013 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-907442 URI |
| 024 | 7 | a https://doi.org/10.1016/j.bone.2012.12.0482 DOI |
| 040 | a (SwePub)liu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Linder, Ceciliau Östergötlands Läns Landsting,Linköpings universitet,Klinisk kemi,Hälsouniversitetet4 aut0 (Swepub:liu)cecli08 |
| 245 | 1 0 | a Isozyme profile and tissue-origin of alkaline phosphatases in mouse serum |
| 264 | 1 | b Elsevier,c 2013 |
| 338 | a print2 rdacarrier | |
| 500 | a Funding Agencies|County Council of Ostergotland in Sweden||National Institutes of Health, USA|DE012889| | |
| 520 | a Mouse serum alkaline phosphatase (ALP) is frequently measured and interpreted in mammalian bone research. However, little is known about the circulating ALPs in mice and their relation to human ALP isozymes and isoforms. Mouse ALP was extracted from liver, kidney, intestine, and bone from vertebra, femur and calvaria tissues. Serum from mixed strains of wild-type (WT) mice and from individual ALP knockout strains were investigated, i.e., Alpl(-/-) (a.k.a. Akp2 encoding tissue-nonspecific ALP or TNALP), Akp3(-/-) (encoding duodenum-specific intestinal ALP or dIALP), and Alpi(-/-) (a.k.a. Akp6 encoding global intestinal ALP or gIALP). The ALP isozymes and isoforms were identified by various techniques and quantified by high-performance liquid chromatography. Results from the WT and knockout mouse models revealed identical bone-specific ALP isoforms (B/I. B1, and B2) as found in human serum, but in addition mouse serum contains the B1x isoform only detected earlier in patients with chronic kidney disease and in human bone tissue. The two murine intestinal isozymes, dIALP and gIALP, were also identified in mouse serum. All four bone-specific ALP isoforms (B/I, B1x, B1, and B2) were identified in mouse bones, in good correspondence with those found in human bones. All mouse tissues, except liver and colon, contained significant ALP activities. This is a notable difference as human liver contains vast amounts of ALP. Histochemical staining, Northern and Western blot analyses confirmed undetectable ALP expression in liver tissue. ALP activity staining showed some positive staining in the bile canaliculi for BALB/c and FVB/N WT mice, but not in C57BI/6 and ICR mice. Taken together, while the main source of ALP in human serum originates from bone and liver, and a small fraction from intestine (andlt;5%), mouse serum consists mostly of bone ALP, including all four isoforms, B/I, B1x, B1, and B2, and two intestinal ALP isozymes dIALP and gIALR We suggest that the genetic nomenclature for the Alpl gene in mice (i.e., ALP liver) should be reconsidered since murine liver has undetectable amounts of ALP activity. These findings should pave the way for the development of user-friendly assays measuring circulating bone-specific ALP in mouse models used in bone and mineral research. | |
| 653 | a Alkaline phosphatase | |
| 653 | a Bone | |
| 653 | a Glycosylation | |
| 653 | a Hypophosphatasia | |
| 653 | a Knockout mice | |
| 653 | a Mineralization | |
| 653 | a MEDICINE | |
| 653 | a MEDICIN | |
| 700 | 1 | a Englund, Ulrikau Linköpings universitet,Cellbiologi,Hälsouniversitetet4 aut0 (Swepub:liu)ulren45 |
| 700 | 1 | a Narisawa, Sonokou Sanford Burnham Medical Research Institute, CA USA4 aut |
| 700 | 1 | a Luis Millan, Joseu Sanford Burnham Medical Research Institute, CA USA4 aut |
| 700 | 1 | a Magnusson, Peru Östergötlands Läns Landsting,Linköpings universitet,Klinisk kemi,Hälsouniversitetet4 aut0 (Swepub:liu)perma28 |
| 710 | 2 | a Linköpings universitetb Klinisk kemi4 org |
| 773 | 0 | t Boned : Elsevierg 53:2, s. 399-408q 53:2<399-408x 8756-3282x 1873-2763 |
| 856 | 4 | u https://europepmc.org/articles/pmc3593980?pdf=render |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-90744 |
| 856 | 4 8 | u https://doi.org/10.1016/j.bone.2012.12.048 |
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