id:"swepub:oai:DiVA.org:liu-98025"
Sökning: id:"swepub:oai:DiVA.org:liu-98025" > The direction of me...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02600naa a2200289 4500 | |
| 001 | oai:DiVA.org:liu-98025 | |
| 003 | SwePub | |
| 008 | 130925s1990 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-980252 URI |
| 024 | 7 | a https://doi.org/10.1126/science.23156952 DOI |
| 040 | a (SwePub)liu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Lee, Julietu Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill, USA4 aut |
| 245 | 1 0 | a The direction of membrane lipid flow in locomoting polymorphonuclear leukocytes |
| 264 | 1 | b American Association for the Advancement of Science,c 1990 |
| 338 | a print2 rdacarrier | |
| 520 | a The objective of this study was to determine the direction of membrane lipid flow in locomoting cells. The plasma membrane of human polymorphonuclear leukocytes was stained with a fluorescent lipid analog dihexadecanoyl indocarbocyanine. A line was photobleached on the cell surface perpendicular to the direction of cell motion. Low-light-level fluorescence microscopy and digital image-processing techniques were used to analyze a series of images taken at short intervals after photobleaching. The bleached line remained visible for about 5 seconds before being erased by diffusional recovery. Examination of fluorescence intensity profiles allowed a comparison to be made between the velocities of line and cell movement. Results indicate that the bleached line moves forward with the same velocity as the cell during locomotion, refuting the retrograde lipid flow model of locomotion. Instead, the plasma membrane lipid appears to move forward according to either the unit movement of membrane or the tank track model of locomotion. | |
| 700 | 1 | a Gustafsson, Mikaelu Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet4 aut0 (Swepub:liu)mikgu97 |
| 700 | 1 | a Magnusson, Karl-Ericu Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet4 aut0 (Swepub:liu)karma55 |
| 700 | 1 | a Jacobson, Kenu Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill, USA4 aut |
| 710 | 2 | a Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill, USAb Medicinsk mikrobiologi4 org |
| 773 | 0 | t Scienced : American Association for the Advancement of Scienceg 247:4947, s. 1229-1233q 247:4947<1229-1233x 0036-8075x 1095-9203 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-98025 |
| 856 | 4 8 | u https://doi.org/10.1126/science.2315695 |
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