Human lipoproteins at model cell membranes: Role of the lipoprotein class on lipid dynamics

Human lipoproteins at model cell membranes : Role of the lipoprotein class on lipid dynamics

Browning, T. K. (author): Department of Pharmacy, Uppsala University, Uppsala, Sweden

Lind, Tania Kjellerup (author): Malmö högskola,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces

Maric, Selma (author): Malmö högskola,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces

Malekkhaiat-Häffner, S. (author): Department of Pharmacy, Uppsala University, Uppsala, Sweden

Fredrikson, G. N. (author): Department of Clinical Sciences, Malmö, Lund University, Malmö, Sweden

Bengtsson, E. (author): Department of Clinical Sciences, Malmö, Lund University, Malmö, Sweden

Malmsten, M. (author): Department of Pharmacy, Uppsala University, Uppsala, Sweden; Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark

Cárdenas, Marité (author): Malmö högskola,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces

(creator_code:org_t)

Related links:: https://doi.org/10.1...; show more...; https://mau.diva-por... (primary) (Raw object); https://www.nature.c...; https://urn.kb.se/re...; https://doi.org/10.1...; show less...

Abstract Subject headings

High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset and development of atherosclerosis; the biggest killer in the western world. Key issues of initial lipoprotein (LP) interactions at cellular membranes need to be addressed including LP deposition and lipid exchange. Here we present a protocol for monitoring the in situ kinetics of lipoprotein deposition and lipid exchange/removal at model cellular membranes using the non-invasive, surface sensitive methods of neutron reflection and quartz crystal microbalance with dissipation. For neutron reflection, lipid exchange and lipid removal can be distinguished thanks to the combined use of hydrogenated and tail-deuterated lipids. Both HDL and LDL remove lipids from the bilayer and deposit hydrogenated material into the lipid bilayer, however, the extent of removal and exchange depends on LP type. These results support the notion of HDL acting as the ‘good’ cholesterol, removing lipid material from lipid-loaded cells, whereas LDL acts as the ‘bad’ cholesterol, depositing lipid material into the vascular wall.

By the author/editor: Browning, T. K.; Lind, Tania Kjel ...; Maric, Selma; Malekkhaiat-Häff ...; Fredrikson, G. N ...; Bengtsson, E.; show more...; Malmsten, M.; Cárdenas, Marité; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biophysics

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