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A hydrogen-bonding ...
A hydrogen-bonding network in mammalian sorbitol dehydrogenase stabilizes the tetrameric state and is essential for the catalytic power
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- Hellgren, Mikko, 1972- (author)
- Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
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- Kaiser, C. (author)
- Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
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- de Haij, S. (author)
- Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
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- Norberg, Å. (author)
- Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
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- Höög, Jan-Olov (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2007-10-20
- 2007
- English.
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In: Cellular and Molecular Life Sciences (CMLS). - : Springer. - 1420-682X .- 1420-9071. ; 64:23, s. 3129-3138
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Subunit interaction in sorbitol dehydrogenase (SDH) has been studied with in vitro and in silico methods identifying a vital hydrogen-bonding network, which is strictly conserved among mammalian SDH proteins. Mutation of one of the residues in the hydrogen-bonding network, Tyr110Phe, abolished the enzymatic activity and destabilized the protein into tetramers, dimers and monomers as judged from gel filtration experiments at different temperatures compared to only tetramers for the wild-type protein below 307 K. The determined equilibrium constants revealed a large difference in Gibbs energy (8 kJ/mol) for the tetramer stability between wild-type SDH and the mutated form Tyr110Phe SDH. The results focus on a network of coupled hydrogen bonds in wild-type SDH that uphold the protein interface, which is specific and favorable to electrostatic, van der Waals and hydrogen-bond interactions between subunits, interactions that are crucial for the catalytic power of SDH.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Alcohol dehydrogenase
- hydrogen-bonding network
- Gibbs energy
- quaternary structure
- sorbitol dehydrogenase
- structural zinc
Publication and Content Type
- ref (subject category)
- art (subject category)
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