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Sökning: id:"swepub:oai:DiVA.org:oru-111543" > Development of a no...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004017naa a2200337 4500
001oai:DiVA.org:oru-111543
003SwePub
008240216s2024 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-1115432 URI
040 a (SwePub)oru
041 a engb eng
042 9 SwePub
072 7a vet2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Matthyssen, Tamarau University of Melbourne, Melbourne Vic, Australia4 aut
2451 0a Development of a novel human oral tissue model of gonorrhoea
264 1b Lippincott Williams & Wilkins,c 2024
338 a print2 rdacarrier
520 a Background: Oropharyngeal Neisseria gonorrhoeae (NG) infections are common, increasing, and have a higher treatment failure compared with other infection sites. Due to antimicrobial resistance, NG has become a global public health threat as available treatments remain scarce. Little is known about where NG colonizes in the oral mucosa and therefore, where antibiotics need to be distributed to cure infection. A recent review also highlighted the lack of oral cell models available for investigating NG infection. We recently started creating an in-vitroco-culture model for NG strains with human oral epithelial cells to understand patterns of NG growth in the mouth and examine antibiotic uptake by oral cell types supporting NG growth.Methods: NG strains were grown on Chocolate agar with IsoVitaleX and in modified Fastidious broth media in optimised conditions. NG colonies were assessed using a colony counter (Scan1200, Interscience technology). In a 2D model, NG were co-cultured with 4 human oral keratinocyte cell lines isolated from different anatomical subsites of theoral cavity. Intra- and extra-cellular NG was quantified, and intracellular spatial distribution was assessed with confocal microscopy and immunocytochemistry. Invasion into 3D spheroids was characterised with penetration depth assessed via histological analysis (haematoxylin and eosin staining) and immunocytochemistry with images taken on a Zeiss Axioscan7 slide scanner or LSM80 0confocal microscope. Real time invasion into spheroids was imaged using a MuviCyte live-cell imaging system. Lastly, host cell viability in response to NG infection was also assessed.Results: We created the first-of-its-kind in-vitro model for NG oral infection demonstrating that it is possible to co-culture NG with oral derived cells. NG survives and infects oral cells in an in vitro setting in both 2D and 3D models. Different strains of NG infected oral cells to significantly different degrees.Conclusion: Our presented model can be used to explore the interactions of NG with oral tissues and to investigate current and new therapeutics against oropharyngeal gonorrhoea. 
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Infektionsmedicin0 (SwePub)302092 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Infectious Medicine0 (SwePub)302092 hsv//eng
7001 a Celentano, Antoniou University of Melbourne, Melbourne Vic, Australia4 aut
7001 a Hocking, Janeu University of Melbourne, Melbourne Vic, Australia4 aut
7001 a Kong, Fabian Yuh Shiongu University of Melbourne, Melbourne Vic, Australia4 aut
7001 a McCullough, Michaelu University of Melbourne, Melbourne Vic, Australia4 aut
7001 a Paolini, Ritau University of Melbourne, Melbourne Vic, Australia4 aut
7001 a Unemo, Magnus,d 1970-u Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län4 aut0 (Swepub:oru)muo
7001 a Williamson, Deborahu University of Melbourne, Melbourne Vic, Australia4 aut
7102 a University of Melbourne, Melbourne Vic, Australiab Institutionen för medicinska vetenskaper4 org
7730 t Sexually Transmitted Diseasesd : Lippincott Williams & Wilkinsg 51:1S, s. S123-S124q 51:1S<S123-S124x 0148-5717x 1537-4521
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-111543

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