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Validation of suita...
Validation of suitable endogenous control genes for expression studies of miRNA in prostate cancer tissues
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- Carlsson, Jessica (author)
- Örebro universitet,Hälsoakademin,Univ Skövde, Skövde, Sweden
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- Helenius, Gisela (author)
- Örebro University Hospital, Örebro, Sweden
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- Karlsson, Mats, 1960- (author)
- Örebro University Hospital, Örebro, Sweden
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- Lubovac, Zelmina (author)
- Syst Biol Res Ctr Bioinfomat, Univ Skövde, Skövde, Sweden
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- Andrén, Ove, 1963- (author)
- Örebro University Hospital, Örebro, Sweden
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- Olsson, Björn (author)
- Syst Biol Res Ctr Bioinfomat, Univ Skövde, Skövde, Sweden
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- Klinga-Levan, Karin (author)
- Syst Biol Res Ctr Tumor Biol, Dept Life Sci, Univ Skövde, Skövde, Sweden
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(creator_code:org_t)
- Elsevier BV, 2010
- 2010
- English.
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In: Cancer Genetics and Cytogenetics. - : Elsevier BV. - 0165-4608 .- 1873-4456. ; 202:2, s. 71-75
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Abstract
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- When performing quantitative polymerase chain reaction analysis, there is a need for correction of technical variation between experiments. This correction is most commonly performed by using endogenous control genes, which are stably expressed across samples, as reference genes for normal expression in a specific tissue. In microRNA (miRNA) studies, two types of control genes are commonly used: small nuclear RNAs and small nucleolar RNAs. In this study, six different endogenous control genes for miRNA studies were investigated in prostate tissue material from the Swedish Watchful Waiting cohort. The stability of the controls was investigated using two different software applications, NormFinder and BestKeeper. RNU24 was the most suitable endogenous control gene for miRNA studies in prostate tissue materials. (C) 2010 Elsevier Inc. All rights reserved.
Keyword
- MEDICINE
- MEDICIN
- Medicine
- Medicin
Publication and Content Type
- ref (subject category)
- art (subject category)
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