id:"swepub:oai:DiVA.org:oru-12889"
Sökning: id:"swepub:oai:DiVA.org:oru-12889" > Expression of Helic...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04118naa a2200493 4500 | |
| 001 | oai:DiVA.org:oru-12889 | |
| 003 | SwePub | |
| 008 | 110103s2010 | |||||||||||000 ||eng| | |
| 009 | oai:prod.swepub.kib.ki.se:121244701 | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-128892 URI |
| 024 | 7 | a https://doi.org/10.1111/j.1523-5378.2010.00786.x2 DOI |
| 024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1212447012 URI |
| 040 | a (SwePub)orud (SwePub)ki | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Kalbina, Irinau Örebro universitet,Akademin för naturvetenskap och teknik4 aut0 (Swepub:oru)ika |
| 245 | 1 0 | a Expression of Helicobacter pylori TonB Protein in Transgenic Arabidopsis thaliana :b toward production of vaccine antigens in plants |
| 264 | c 2010-09-07 | |
| 264 | 1 | b John Wiley & Sons,c 2010 |
| 338 | a electronic2 rdacarrier | |
| 500 | a This is a pre-print article. | |
| 520 | a Background: The aim of this study was to produce a recombinant version of the highly antigenic Helicobacter pylori TonB (iron-dependent siderophore transporter protein HP1341) in transgenic plants as a candidate oral vaccine antigen. Materials and Methods: Using Agrobacterium-mediated gene transfer, we introduced three different constructs of the tonB gene into the genome of the model plant Arabidopsis thaliana. We investigated transgene insertion by PCR, produced TonB antibodies for analysis of the production of the recombinant protein in plants, verified the identity of the protein produced by mass spectrometry analysis, and analyzed the number of genetic inserts in the plants by Southern blotting. Results: Three different constructs of the expression cassette (full-length tonB, tonB truncated in the 5' end removing the codons for a transmembrane helix, and the latter construct with codons for the endoplasmic reticulum SEKDEL retention signal added to the 3' end) were used to find the most effective way to express the TonB antigen. Production of TonB protein was detected in plants transformed with each of the constructs, confirmed by both Western blotting and mass spectrometry analysis. No considerable differences in protein expression from the three different constructs were observed. The protein concentration in the plants was at least 0.05% of the total soluble proteins. Conclusions: The Helicobacter pylori TonB protein can be produced in Arabidopsis thaliana plants in a form that is recognizable by rabbit anti-TonB antiserum. These TonB-expressing plants are highly suitable for animal studies of oral adminstration as a route for immunization against Helicobacter infections. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 653 | a edible vaccines | |
| 653 | a Helicobacter pylori | |
| 653 | a HP1341 | |
| 653 | a subunit vaccine | |
| 653 | a TonB | |
| 653 | a transgenic plants | |
| 653 | a Biochemistry | |
| 653 | a Biokemi | |
| 700 | 1 | a Engstrand, Larsu Karolinska Institutet4 aut |
| 700 | 1 | a Andersson, Sörenu Örebro University Hospital, Örebro, Sweden; Dept Bacteriol, Swedish Inst Infect Dis Control SMI, Solna, Sweden4 aut0 (Swepub:oru)sean |
| 700 | 1 | a Strid, Åkeu Örebro universitet,Akademin för naturvetenskap och teknik4 aut0 (Swepub:oru)aesd |
| 710 | 2 | a Örebro universitetb Akademin för naturvetenskap och teknik4 org |
| 773 | 0 | t Helicobacterd : John Wiley & Sonsg 15:5, s. 430-437q 15:5<430-437x 1083-4389x 1523-5378 |
| 856 | 4 | u https://oru.diva-portal.org/smash/get/diva2:383450/FULLTEXT01.pdfx primaryx Raw objecty fulltext:postprint |
| 856 | 4 | u http://oru.diva-portal.org/smash/get/diva2:383450/FULLTEXT01 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-12889 |
| 856 | 4 8 | u https://doi.org/10.1111/j.1523-5378.2010.00786.x |
| 856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:121244701 |
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