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Detection of Yersinia enterocolitica in food by PCR amplification

Nilsson, Anders (author)
National Food Administration, Swedish University of Agricultural Sciences, Uppsala
Lambertz, Susanne (author)
National Food Administration, Swedish University of Agricultural Sciences, Uppsala
Stålhandske, Per (author)
Department of Food Hygiene, Swedish University of Agricultural Sciences, Uppsala
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Norberg, Per (author)
National Food Administration, Swedish University of Agricultural Sciences, Uppsala
Danielsson-Tham, Marie-Louise, 1947- (author)
Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden
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 (creator_code:org_t)
Oxon, United Kingdom : Blackwell Publishing, 1998
1998
English.
In: Letters in Applied Microbiology. - Oxon, United Kingdom : Blackwell Publishing. - 0266-8254 .- 1472-765X. ; 26, s. 140-144
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • A polymerase chain reaction (PCR) assay was developed for detection ofpathogenic, virulent strains of Yersinia enterocolitica. By using both virulence loci virFand ail as markers for pathogenicity, detection of species with a virulence factor present waspossible. DNA preparation in the presence of hexadecyl trimethy ammonium bromide(CTAB) was followed by two 44 cycle amplification reactions, one for each of themarkers. As few as 102Y. enterocolitica cells were detected in ground pork in thepresence of 105–106bacteria of other species. The described PCR assay providesa sensitive robust assay for the detection of virulent Y. enterocolitica in food.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)

Keyword

Måltidskunskap
Culinary Arts and Meal Science

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Nilsson, Anders
Lambertz, Susann ...
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Norberg, Per
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MEDICAL AND HEALTH SCIENCES
MEDICAL AND HEAL ...
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Örebro University

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