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Integrin Dependent ...
Integrin Dependent RhoB Activation Assay Using Leukocytes
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- Lehtonen, Janne (författare)
- Department of Experimental Medical Science, Lund University, Lund, Sweden
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- Potrzebowska, Katarzyna (författare)
- Department of Experimental Medical Science, Lund University, Lund, Sweden
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- Samuelsson, Malin (författare)
- Department of Experimental Medical Science, Lund University, Lund, Sweden
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- Svensson, Lena, 1973- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Department of Experimental Medical Science, Lund University, Lund, Sweden
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(creator_code:org_t)
- Bio-Protocol, 2018
- 2018
- Engelska.
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Ingår i: Bio-protocol. - : Bio-Protocol. - 2331-8325. ; 8:23
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.2...
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Abstract
Ämnesord
Stäng
- To be able to migrate, leukocyte needs to re-use its adhesion molecules to move forward. These adhesion molecules are called integrins and are intracellularly transported via endocytosis and exocytosis in order to translocate to a new site on the cell membrane. The intracellular transportation is regulated by different small GTPases including RhoB. Here we describe an activation assay of RhoB in leukocytes migrating on ICAM-1Fc coated dishes using commercially available Rhoteikin coated agarose beads. Although this is a specific protocol for LFA-1 induced RhoB activation, it can also be used for RhoB activation induced by other soluble and insoluble factors.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- Leukocytes
- LFA-1
- RhoB
- Small GTPases
- Rhoteikin
- Pull-down
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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