Search: id:"swepub:oai:DiVA.org:oru-93496" >
Differential effect...
Differential effects of estradiol and progesterone on human T cell activation in vitro
-
- Papapavlou, Georgia (author)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten
-
- Hellberg, Sandra (author)
- Linköpings universitet,Bioinformatik,Tekniska fakulteten
-
- Raffetseder, Johanna (author)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten
-
show more...
-
- Brynhildsen, Jan, 1962- (author)
- Linköpings universitet,Örebro universitet,Institutionen för medicinska vetenskaper,Department of Obstetrics and Gynecology, and Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden; Department of Obstetrics and Gynecology, Faculty of Medicine, Örebro University, Örebro, Sweden,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,Region Östergötland, Kvinnokliniken US,Orebro Univ, Sweden
-
- Gustafsson, Mika (author)
- Linköpings universitet,Bioinformatik,Tekniska fakulteten
-
- Jenmalm, Maria (author)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten
-
- Ernerudh, Jan (author)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Region Östergötland, Klinisk immunologi och transfusionsmedicin
-
show less...
-
(creator_code:org_t)
- 2021-07-26
- 2021
- English.
-
In: European Journal of Immunology. - : Wiley-VCH Verlagsgesellschaft. - 0014-2980 .- 1521-4141. ; 51:10, s. 2430-2440
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://onlinelibrar...
-
https://liu.diva-por... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- Estradiol (E2) and progesterone (P4) are steroid hormones important for the regulation of immune responses during pregnancy. Their increasing levels coincide with an improvement of T cell-mediated diseases such as multiple sclerosis (MS). Although immune-endocrine interactions are involved in this phenomenon, the relative contribution of hormones is not known. We here report a direct comparison of E2- and P4-mediated effects on human CD4+ T cells, key cells in immune regulation. T cells were stimulated to obtain different activation levels and exposed to a broad range of hormone concentrations. Activation level was assessed by CD69/CD25 expression by flow cytometry, and secreted proteins (n = 196) were measured in culture supernatants using proximity extension assay and electrochemiluminescence immunoassay. We found that in low activated cells, pregnancy-relevant E2 concentrations increased activation and the secretion of several immune- and inflammation-related proteins. P4, on the other hand, showed a biphasic pattern, where serum-related concentrations upregulated activation and protein secretion while placenta-relevant concentrations induced a prominent dampening irrespective of the initial activation level. Our results demonstrate the importance of P4 as a major hormone in the immune modulation of T cells during pregnancy and emphasize the need to further evaluate its potency in the treatment of diseases like MS.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
Keyword
- CD4+ T cell activation
- estrogen
- human
- progesterone
- proteomics
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database