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Graphene oxide is d...
Graphene oxide is degraded by neutrophils and the degradation products are non-genotoxic
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- Mukherjee, Sourav P. (författare)
- Karolinska Institutet, Sweden
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- Gliga, Anda R. (författare)
- Karolinska Institutet
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- Lazzaretto, Beatrice (författare)
- Karolinska Institutet
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- Brandner, Birgit (författare)
- Karolinska Institutet,RISE,Yta, process och formulering
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- Fielden, Matthew (författare)
- KTH,Tillämpad fysik
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- Vogt, Carmen (författare)
- KTH,Biomedicinsk fysik och röntgenfysik
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- Newman, Leon (författare)
- University of Manchester, UK
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- Rodrigues, Artur F. (författare)
- University of Manchester, UK
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- Shao, Wenting (författare)
- University of Pittsburgh, USA
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- Fournier, Philip M. (författare)
- University of Pittsburgh, USA
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- Toprak, Muhammet Sadaka (författare)
- KTH,Biomedicinsk fysik och röntgenfysik
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- Star, Alexander (författare)
- University of Pittsburgh, USA
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- Kostarelos, Kostas (författare)
- University of Manchester, UK
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- Bhattacharya, Kunal (författare)
- Karolinska Institutet, Sweden
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- Fadeel, Bengt (författare)
- Karolinska Institutet, Sweden
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(creator_code:org_t)
- 2018
- 2018
- Engelska.
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Ingår i: Nanoscale. - : Royal Society of Chemistry (RSC). - 2040-3364 .- 2040-3372. ; 10:3, s. 1180-1188
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Neutrophils were previously shown to digest oxidized carbon nanotubes through a myeloperoxidase (MPO)-dependent mechanism, and graphene oxide (GO) was found to undergo degradation when incubated with purified MPO, but there are no studies to date showing degradation of GO by neutrophils. Here we produced endotoxin-free GO by a modified Hummers' method and asked whether primary human neutrophils stimulated to produce neutrophil extracellular traps or activated to undergo degranulation are capable of digesting GO. Biodegradation was assessed using a range of techniques including Raman spectroscopy, transmission electron microscopy, atomic force microscopy, and mass spectrometry. GO sheets of differing lateral dimensions were effectively degraded by neutrophils. As the degradation products could have toxicological implications, we also evaluated the impact of degraded GO on the bronchial epithelial cell line BEAS-2B. MPO-degraded GO was found to be non-cytotoxic and did not elicit any DNA damage. Taken together, these studies have shown that neutrophils can digest GO and that the biodegraded GO is non-toxic for human lung cells.
Ämnesord
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
Nyckelord
- Atomic force microscopy
- Biodegradation
- Carbon
- Cell culture
- Degradation
- High resolution transmission electron microscopy
- Mass spectrometry
- Transmission electron microscopy
- Yarn
- Degradation products
- Epithelial cells
- Graphene oxides
- Human lung cells
- Human neutrophil
- Lateral dimension
- Myeloperoxidase
- Oxidized carbons
- Graphene
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Mukherjee, Soura ...
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Gliga, Anda R.
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Lazzaretto, Beat ...
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Brandner, Birgit
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Fielden, Matthew
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Vogt, Carmen
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visa fler...
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Newman, Leon
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Rodrigues, Artur ...
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Shao, Wenting
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Fournier, Philip ...
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Toprak, Muhammet ...
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Star, Alexander
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Kostarelos, Kost ...
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Bhattacharya, Ku ...
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Fadeel, Bengt
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Fysik
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och Annan fysik
- Artiklar i publikationen
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Nanoscale
- Av lärosätet
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RISE
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Kungliga Tekniska Högskolan
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Karolinska Institutet