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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003035naa a2200385 4500
001oai:DiVA.org:sh-15838
003SwePub
008120309s2001 | |||||||||||000 ||eng|
009oai:prod.swepub.kib.ki.se:1927509
024a https://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-158382 URI
024a https://doi.org/10.1093/glycob/11.12.10092 DOI
024a http://kipublications.ki.se/Default.aspx?queryparsed=id:19275092 URI
040 a (SwePub)shd (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
100a Li, J J4 aut
2451 0a Glycine is a common substituent of the inner core in Haemophilus influenzae lipopolysaccharide
264 1b Oxford University Press (OUP),c 2001
338 a print2 rdacarrier
520 a A survey of both typeable and nontypeable strainsof Haemophilus influenzae indicated that they contain glycine (Gly) in their lipopolysaccharide (LPS). Significant amounts (30-250 pmol Gly/mug LPS) were determined by high-performance anion-exchange chromatography using pulsed amperometric detection after treatment of the LPS with mild alkali. Oligosaccharides obtained from LPS after mild acid hydrolysis and gel filtration chromatography were investigated by electrospray ionization mass spectrometry (ESI-MS) and capillary electrophoresis (CE) ESI-MS. In all cases, molecular ions corresponding to the major glycoforms were identified and were accompanied by ions differing by 57 Da, thus indicating the presence of glycine. The position of glycine in these glycoforms was determined by CE-ESI-MS/MS analyses. It was found that, depending on strain, glycine can substitute each of the heptoses of the inner-core element, L-alpha-D-Hepp-(1-->2)-[PEtn-->6]-L-alpha-D-Hepp-(1-->3)-L-alpha-D-Hepp- (1-->5)-alpha-Kdo of H. influenzae LPS as well as Kdo. In some strains, mixtures of monosubstituted Gly-containing glycoforms having different substitution patterns were identified.
650 7a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
700a Bauer, S H J4 aut
700a Månsson, Martinu Södertörns högskola,Avdelning Naturvetenskap4 aut0 (Swepub:sh)SHMNMN
700a Moxon, E R4 aut
700a Richards, J C4 aut
700a Schweda, Elke K Hu Karolinska Institutet,Södertörns högskola,Avdelning Naturvetenskap4 aut0 (Swepub:sh)SHEESA
710a Södertörns högskolab Avdelning Naturvetenskap4 org
773t Glycobiologyd : Oxford University Press (OUP)g 11:12, s. 1009-1015q 11:12<1009-1015x 0959-6658x 1460-2423
856u https://academic.oup.com/glycob/article-pdf/11/12/1009/9806204/111009.pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-15838
8564 8u https://doi.org/10.1093/glycob/11.12.1009
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:1927509

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