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  • Nordin, Joel Z.Karolinska Institutet (author)

Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties

  • Article/chapterEnglish2015

Publisher, publication year, extent ...

  • Elsevier BV,2015
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:su-118282
  • https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-118282URI
  • https://doi.org/10.1016/j.nano.2015.01.003DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-104379URI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:131270848URI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Extracellular vesicles (EVs) are natural nanoparticles that mediate intercellular transfer of RNA and proteins and are of great medical interest; serving as novel biomarkers and potential therapeutic agents. However, there is little consensus on the most appropriate method to isolate high-yield and high-purity EVs from various biological fluids. Here, we describe a systematic comparison between two protocols for EV purification: ultrafiltration with subsequent liquid chromatography (UF-LC) and differential ultracentrifugation (UC). A significantly higher EV yield resulted from UF-LC as compared to UC, without affecting vesicle protein composition. Importantly, we provide novel evidence that, in contrast to UC-purified EVs, the biophysical properties of UF-LC-purified EVs are preserved, leading toadifferent in vivo biodistribution, with less accumulation in lungs. Finally, we show that UF-LC is scalable and adaptable for EV isolation from complex media types such as stem cell media, which is of huge significance for future clinical applications involving EVs.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Lee, YiKarolinska Institutet (author)
  • Vader, Pieter (author)
  • Mäger, Imre (author)
  • Johansson, Henrik J.Karolinska Institutet (author)
  • Heusermann, Wolf (author)
  • Wiklander, Oscar P. B.Karolinska Institutet (author)
  • Hällbrink, MattiasStockholms universitet,Institutionen för neurokemi(Swepub:su)mhall (author)
  • Seow, Yiqi (author)
  • Bultema, Jarred J. (author)
  • Gilthorpe, JonathanUmeå universitet,Umeå centrum för molekylär medicin (UCMM)(Swepub:umu)jogi0002 (author)
  • Davies, Tim (author)
  • Fairchild, Paul J. (author)
  • Gabrielsson, SusanneKarolinska Institutet (author)
  • Meisner-Kober, Nicole C. (author)
  • Lehtio, JanneKarolinska Institutet (author)
  • Smith, C. I. EdvardKarolinska Institutet (author)
  • Wood, Matthew J. A. (author)
  • Andaloussi, Samir E. L.Karolinska Institutet (author)
  • Karolinska InstitutetInstitutionen för neurokemi (creator_code:org_t)

Related titles

  • In:Nanomedicine: Elsevier BV11:4, s. 879-8831549-96341549-9642

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