Search: id:"swepub:oai:DiVA.org:su-158299" >
The polymorphism rs...
The polymorphism rs2480258 within CYP2E1 is associated with different rates of acrylamide metabolism in vivo in humans
-
Pellè, Lucia (author)
-
- Carlsson, Henrik (author)
- Stockholms universitet,Institutionen för miljövetenskap och analytisk kemi
-
Cipollini, Monica (author)
-
show more...
-
Bonotti, Alessandra (author)
-
Foddis, Rudy (author)
-
Cristaudo, Alfonso (author)
-
Romei, Cristina (author)
-
Elisei, Rossella (author)
-
Gemignani, Federica (author)
-
- Törnqvist, Margareta (author)
- Stockholms universitet,Institutionen för miljövetenskap och analytisk kemi
-
Landi, Stefano (author)
-
show less...
-
(creator_code:org_t)
- 2018-05-10
- 2018
- English.
-
In: Archives of Toxicology. - : Springer Science and Business Media LLC. - 0340-5761 .- 1432-0738. ; 92:6, s. 2137-2140
- Related links:
-
https://arpi.unipi.i...
-
show more...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- In a recent study, we demonstrated that the variant allele of rs2480258 within intron VIII of CYP2E1 is associated with reduced levels of mRNA, protein, and enzyme activity. CYP2E1 is the most important enzyme in the metabolism of acrylamide (AA) by operating its oxidation into glycidamide (GA). AA occurs in food, is neurotoxic and classified as a probable human carcinogen. The goal of the present study was to further assess the role of rs2480258 by measuring the rate of AA > GA biotransformation in vivo. In blood samples from a cohort of 120 volunteers, the internal doses of AA and GA were assessed by AA and GA adducts to hemoglobin (Hb) measured by mass spectrometry. The rate of biotransformation was assessed by calculating the GA-Hb/AA-Hb ratio. To maximize the statistical power, 60 TT was compared to 60 CC-homozygotes and the results showed that TT homozygotes had a statistically significant reduced rate of biotransformation. Present results reinforced the notion that T-allele of rs2480258 is a marker of low functional activity of CYP2E1. Moreover, we studied the role of polymorphisms (SNPs) within glutathione-S-transferases (GSTs) enzymes and epoxide hydrolase (EPHX), verifying previous findings that SNPs within GSTs and EPHX influence the metabolism rate.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
Keyword
- CYP2E1
- Xenobiotics metabolism
- Functional polymorphism
- rs2480258
- Acrylamide
- Glycidamide
- Hemoglobin adducts
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Pellè, Lucia
-
Carlsson, Henrik
-
Cipollini, Monic ...
-
Bonotti, Alessan ...
-
Foddis, Rudy
-
Cristaudo, Alfon ...
-
show more...
-
Romei, Cristina
-
Elisei, Rossella
-
Gemignani, Feder ...
-
Törnqvist, Marga ...
-
Landi, Stefano
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Pharmaceutical S ...
- Articles in the publication
-
Archives of Toxi ...
- By the university
-
Stockholm University