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A simple pressure-assisted method for MicroED specimen preparation

Zhao, Jingjing, 1988- (author)
Stockholms universitet,Institutionen för material- och miljökemi (MMK)
Xu, Hongyi (author)
Stockholms universitet,Institutionen för material- och miljökemi (MMK)
Lebrette, Hugo (author)
Stockholms universitet,Institutionen för biokemi och biofysik
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Carroni, Marta (author)
Stockholms universitet,Institutionen för biokemi och biofysik
Taberman, Helena (author)
Högbom, Martin (author)
Stockholms universitet,Institutionen för biokemi och biofysik
Zou, Xiaodong (author)
Stockholms universitet,Institutionen för material- och miljökemi (MMK)
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 (creator_code:org_t)
English.
Related links:
https://urn.kb.se/re...
  • Other publication (other academic/artistic)
Abstract Subject headings
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  • Micro-crystal electron diffraction (MicroED) has shown great potential for structure determination of macromolecular crystals too small for X-ray diffraction. However, specimen preparation remains a major bottleneck. Here, we report a simple method for preparing MicroED specimens, named Preassis, in which excess liquid is removed through an EM grid with the assistance of pressure. We show the ice thicknesses can be controlled by tuning the pressure in combination with EM grids with appropriate hole sizes. Importantly, Preassis can handle a wide range of protein crystals grown in various buffer conditions including those with high viscosity, as well as samples with low crystal contents. Preassis is a simple and universal method for MicroED specimen preparation, and will significantly broaden the applications of MicroED. 

Subject headings

NATURVETENSKAP  -- Biologi -- Strukturbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Structural Biology (hsv//eng)
NATURVETENSKAP  -- Kemi -- Fysikalisk kemi (hsv//swe)
NATURAL SCIENCES  -- Chemical Sciences -- Physical Chemistry (hsv//eng)

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