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Oxalate Oxidase for In Situ H2O2-Generation in Unspecific Peroxygenase-Catalysed Drug Oxyfunctionalisations
- Romero, Elvira (author)
-
- Johansson, Magnus J. (author)
- Stockholms universitet,Institutionen för organisk kemi,AstraZeneca, Sweden
- Cartwright, Jared (author)
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- Grogan, Gideon (author)
- Hayes, Martin A. (author)
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- (creator_code:org_t)
- 2022-08-25
- 2022
- English.
- In: Angewandte Chemie International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 61:39
- Journal article (peer-reviewed)
Abstract
Subject headings
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- H2O2-driven enzymes are of great interest for industrial biotransformations. Herein, we show for the first time that oxalate oxidase (OXO) is an efficient in situ source of H2O2 for one of these biocatalysts, which is known as unspecific peroxygenase (UPO). OXO is reasonably robust, produces only CO2 as a by-product and uses oxalate as a cheap sacrificial electron donor. UPO has significant potential as an industrial catalyst for selective C−H oxyfunctionalisations, as we confirm herein by testing a diverse drug panel using miniaturised high-throughput assays and mass spectrometry. 33 out of 64 drugs were converted in 5 μL-scale reactions by the UPO with OXO (conversion >70 % for 11 drugs). Furthermore, oxidation of the drug tolmetin was achieved on a 50 mg scale (TONUPO 25 664) with 84 % yield, which was further improved via enzyme immobilization. This one-pot approach ensures adequate H2O2 levels, enabling rapid access to industrially relevant molecules that are difficult to obtain by other routes.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Biocatalysis
- Drug Late-Stage Functionalisation
- H2O2-Generation
- High-Throughput Screening
- Oxidoreductases
Publication and Content Type
- ref (subject category)
- art (subject category)
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