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Structural and immu...
Structural and immunochemical relationship between the O-antegenic polysaccharides from the enteroaggregative Escherichia coli strain 396/C1 and Escherichia coli O126
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- Larsson, Andreas (författare)
- Stockholms universitet,Institutionen för organisk kemi
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- Urbina, Felipe (författare)
- bKarolinska Institute, Department of Microbiology, Pathology and Immunology, Division of Clinical Bacteriology
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- Yang, Zhennai (författare)
- Stockholms universitet,Institutionen för organisk kemi
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- Weintraub, Andrej (författare)
- Karolinska Institutet
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- Widmalm, Göran (författare)
- Stockholms universitet,Institutionen för organisk kemi
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(creator_code:org_t)
- Elsevier, 2004
- 2004
- Engelska.
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Ingår i: Carbohydrate Research. - : Elsevier. - 0008-6215. ; 339:8, s. 1491-1496
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- The structure of the O-antigen polysaccharide (PS) from the enteroaggregative Escherichia coli strain 396/C-1 has been determined. Sugar and methylation analyses together with 1H and 13C NMR spectroscopy were the main methods used. Inter-residue correlations were determined by 1H,1H-NOESY, 1H,13C-heteronuclear multiple-bond correlation and dipole–dipole cross-correlated relaxation experiments. The PS is composed of pentasaccharide repeating units with the following structure: Full-size image (2K) Analysis of NMR data reveals that on average the PS consists of 13 repeating units and indicates that the biological repeating unit contains an N-acetylglucosamine residue at its reducing end. This structure is different to that reported for the O-antigen polysaccharide from E. coli O126. Monospecific anti-E. coli O126 rabbit serum from The International Escherichia and Klebsiella Centre did not distinguish between the E. coli strain 396/C-1 and the E. coli O126 reference strain, neither in slide agglutination nor in an indirect enzyme immunoassay. Subsequent successful serotyping of the E. coli strain 396/C-1 showed it to be E. coli O126:K+:H27.
Ämnesord
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Nyckelord
- Escherichia coli; Enteroaggregative; Serology; Lipopolysaccharide; NMR
- Organic chemistry
- Organisk kemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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