Low doses of γ-radiation induce consistent protein expression changes in human leukocytes

Skiöld, Sara (author): Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik

Harms-Ringdahl, Mats (author): Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik

Haghdoost, Siamak (author): Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik

(creator_code:org_t)

Inderscience Publishers, 2011
2011
English.
In: International Journal of Low Radiation. - Inderscience Publishers. - 1477-6545 .- 1741-9190. ; 8:5/6, s. 374-387

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; show less...

Abstract Subject headings

Twenty percent of cancer patients experience adverse effects after radiotherapy. The therapeutic doses are adjusted to the most sensitive individuals, resulting in a suboptimal dose for many patients. At present there is no screening system available to predict individual radiosensitivity. The main aim of this study is to investigate differences in protein expression pathways induced by low doses of radiation in whole blood obtained from radiosensitive and non–radiosensitive patients. To address this aim, a protocol for exposure condition, dose and analysis of whole blood was developed. The conditions for handling blood samples were optimised. The optimal doses and post irradiation conditions were determined. We found that 1 mGy and 150 mGy significantly changed protein expression in leukocytes collected from the two donors. The result shows that the protocol developed is suitable for characterisation of proteomic profiles associated with low dose exposure of leukocytes.

By the author/editor: Skiöld, Sara; Becker, Susanne; Hellman, Ulf; Auer, Gert; Näslund, Ingemar; Harms-Ringdahl, ...; show more...; Haghdoost, Siama ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Cell Biology

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Other Biological ...

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