Sökning: id:"swepub:oai:DiVA.org:umu-10418" >
Acquisition of Cisp...
Acquisition of Cisplatin-resistance in Malignant Mesothelioma Cells Abrogates Na,K(+),2Cl(-;)-cotransport Activity and Cisplatin-induced Early Membrane Blebbing
-
- Janson, Veronica (författare)
- Umeå universitet,Klinisk kemi
-
- Andersson, Britta (författare)
- Umeå universitet,Klinisk kemi
-
- Behnam-Motlagh, Parviz (författare)
- Umeå universitet,Klinisk kemi
-
visa fler...
-
- Engström, Karl-Gunnar (författare)
- Umeå universitet,Kirurgi
-
- Henriksson, Roger (författare)
- Umeå universitet,Onkologi
-
- Grankvist, Kjell (författare)
- Umeå universitet,Klinisk kemi
-
visa färre...
-
(creator_code:org_t)
- S. Karger, 2008
- 2008
- Engelska.
-
Ingår i: Cellular Physiology and Biochemistry. - : S. Karger. - 1015-8987 .- 1421-9778. ; 22:1-4, s. 45-56
- Relaterad länk:
-
https://www.karger.c...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- AIMS: Resistance mechanisms are important limiting factors in the treatment of solid malignancies with cis-diamminedichloroplatinum(II) (cisplatin). To gain further understanding of the effects of acquired cisplatin-resistance, we compared a human malignant pleural mesothelioma cell line (p31) to a sub-line (p31res1.2) with acquired cisplatin-resistance.METHODS AND RESULTS: The role of Na(+),K(+),2Cl(-)-cotransport (NKCC1) activity in cisplatin-induced morphological changes and acquired cisplatin-resistance was investigated in a time-resolved manner. Acquisition of cisplatin-resistance resulted in markedly reduced NKCC1 activity, absence of cisplatin-induced early membrane blebbing, and increased basal caspase-3 activity. At equitoxic cisplatin concentrations, P31res1.2 cells had a faster activation of caspase-3 than P31 cells, but the end-stage cytotoxicity and number of cells with DNA fragmentation was similar. Bumetanide inhibition of NKCC1 activity in P31 cells repressed cisplatin-induced early-phase membrane blebbing but did not increase P31 cell resistance to cisplatin.CONCLUSIONS: Together, these results suggest that active NKCC1 was necessary for cisplatin-induced early membrane blebbing of P31 cells, but not for cisplatin-resistance. Thus, acquisition of cisplatin-resistance can affect mechanisms that have profound effects on cisplatin-induced morphological changes but are not necessary for the subsequent progression to apoptosis.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Apoptosis
- Caspase-3
- Cisplatin
- Malignant mesothelioma
- Membrane blebbing
- Na
- K
- 2Cl--cotransport
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas