Expansion of the Chlamydia trachomatis inclusion does not require bacterial replication

Engström, Patrik (author): Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Molekylär Infektionsmedicin, Sverige (MIMS),Umeå Centre for Microbial Research (UCMR)

Bergström, Malin (author): Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)

Alfaro, Astrid C. (author)

Krishnan, K. Syam (author): Umeå universitet,Umeå Centre for Microbial Research (UCMR),Kemiska institutionen

Bahnan, Wael (author): Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Molekylär Infektionsmedicin, Sverige (MIMS),Umeå Centre for Microbial Research (UCMR)

Almqvist, Fredrik (author): Umeå universitet,Umeå Centre for Microbial Research (UCMR),Kemiska institutionen

Bergström, Sven (author): Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Molekylär Infektionsmedicin, Sverige (MIMS),Umeå Centre for Microbial Research (UCMR)

(creator_code:org_t)

Elsevier BV, 2015
2015
English.
In: International Journal of Medical Microbiology. - : Elsevier BV. - 1438-4221 .- 1618-0607. ; 305:3, s. 378-382

Related links:: https://doi.org/10.1...; show more...; https://urn.kb.se/re...; https://doi.org/10.1...; show less...

Abstract Subject headings

Chlamydia trachomatis replication takes place inside of a host cell, exclusively within a vacuole known as the inclusion. During an infection, the inclusion expands to accommodate the increasing numbers of C. trachomatis. However, whether inclusion expansion requires bacterial replication and/or de novo protein synthesis has not been previously investigated in detail. Therefore, using a chemical biology approach, we herein investigated C. trachomatis inclusion expansion under varying conditions in vitro. Under normal cell culture conditions, inclusion expansion correlated with C trachomatis replication. When bacterial replication was inhibited using KSK120: an inhibitor that targets C. trachomatis glucose metabolism, inclusions expanded even in the absence of bacterial replication. In contrast, when bacterial protein synthesis was inhibited using chloramphenicol, expansion of inclusions was blocked. Together, these data suggest that de novo protein synthesis is necessary, whereas bacterial replication is dispensable for C trachomatis inclusion expansion. (C) 2015 The Authors. Published by Elsevier GmbH.

By the author/editor: Engström, Patrik; Bergström, Malin; Alfaro, Astrid C ...; Krishnan, K. Sya ...; Bahnan, Wael; Almqvist, Fredri ...; show more...; Bergström, Sven; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Microbiology in ...

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