Sökning: id:"swepub:oai:DiVA.org:umu-114202" >
Normalization of qR...
Normalization of qRT-PCR data : the necessity of adopting a systematic, experimental conditions-specific, validation of references
-
Guenin, Stephanie (författare)
-
- Mauriat, Melanie (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology
-
Pelloux, Jerome (författare)
-
visa fler...
-
Van Wuytswinkel, Olivier (författare)
-
- Bellini, Catherine (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology,National Institute of Agricultural Research (INRA)
-
Gutierrez, Laurent (författare)
-
visa färre...
-
(creator_code:org_t)
-
- 2009-01-06
- 2009
- Engelska.
-
Ingår i: Journal of Experimental Botany. - : Oxford University Press (OUP). - 0022-0957 .- 1460-2431. ; 60:2, s. 487-493
- Relaterad länk:
-
http://<Go to ISI>:/...
-
visa fler...
-
https://academic.oup...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://res.slu.se/i...
-
visa färre...
Abstract
Ämnesord
Stäng
- Quantitative RT-PCR (reverse transcription polymerase chain reaction, also known as qRT-PCR or real-time RT-PCR) has been used in large proportions of transcriptome analyses published to date. The accuracy of the results obtained by this method strongly depends on accurate transcript normalization using stably expressed genes, known as references. Statistical algorithms have been developed recently to help validate reference genes but, surprisingly, this robust approach is under-utilized in plants. Instead, putative ’housekeeping’ genes tend to be used as references without any proper validation. The concept of normalization in transcript quantification is introduced here and the factors affecting its reliability in qRT-PCR are discussed in an attempt to convince molecular biologists, and non-specialists, that systematic validation of reference genes is essential for producing accurate, reliable data in qRT-PCR analyses, and thus should be an integral component of them.
Ämnesord
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Skogsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Forest Science (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas