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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04353naa a2200529 4500 | |
| 001 | oai:DiVA.org:umu-150725 | |
| 003 | SwePub | |
| 008 | 180829s2018 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1507252 URI |
| 024 | 7 | a https://doi.org/10.1186/s12879-018-3218-22 DOI |
| 040 | a (SwePub)umu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Birdsell, D. N.4 aut |
| 245 | 1 0 | a Coinfections identified from metagenomic analysis of cervical lymph nodes from tularemia patients |
| 264 | c 2018-07-11 | |
| 264 | 1 | b BMC,c 2018 |
| 338 | a electronic2 rdacarrier | |
| 520 | a Background: Underlying coinfections may complicate infectious disease states but commonly go unnoticed because an a priori clinical suspicion is usually required so they can be detected via targeted diagnostic tools. Shotgun metagenomics is a broad diagnostic tool that can be useful for identifying multiple microbes simultaneously especially if coupled with lymph node aspirates, a clinical matrix known to house disparate pathogens. The objective of this study was to analyze the utility of this unconventional diagnostic approach (shotgun metagenomics) using clinical samples from human tularemia cases as a test model. Tularemia, caused by the bacterium Francisella tularensis, is an emerging infectious disease in Turkey. This disease commonly manifests as swelling of the lymph nodes nearest to the entry of infection. Because swollen cervical nodes are observed from many different types of human infections we used these clinical sample types to analyze the utility of shotgun metagenomics.Methods: We conducted an unbiased molecular survey using shotgun metagenomics sequencing of DNA extracts from fine-needle aspirates of neck lymph nodes from eight tularemia patients who displayed protracted symptoms. The resulting metagenomics data were searched for microbial sequences (bacterial and viral).Results: F. tularensis sequences were detected in all samples. In addition, we detected DNA of other known pathogens in three patients. Both Hepatitis B virus (HBV) and Human Parvovirus B-19 were detected in one individual and Human Parvovirus B-19 alone was detected in two other individuals. Subsequent PCR coupled with Sanger sequencing verified the metagenomics results. The HBV status was independently confirmed via serological diagnostics, despite evading notice during the initial assessment.Conclusion: Our data highlight that shotgun metagenomics of fine-needle lymph node aspirates is a promising clinical diagnostic strategy to identify coinfections. Given the feasibility of the diagnostic approach demonstrated here, further steps to promote integration of this type of diagnostic capability into mainstream clinical practice are warranted. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Infektionsmedicin0 (SwePub)302092 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Infectious Medicine0 (SwePub)302092 hsv//eng |
| 653 | a Coinfections | |
| 653 | a Concurrent infections | |
| 653 | a Tularemia | |
| 653 | a Francisella tularensis | |
| 653 | a Metagenomics | |
| 653 | a Fine-needle lymph node aspirate | |
| 700 | 1 | a Özsürekci, Y.4 aut |
| 700 | 1 | a Rawat, A.4 aut |
| 700 | 1 | a Aycan, A. E.4 aut |
| 700 | 1 | a Mitchell, C. L.4 aut |
| 700 | 1 | a Sahl, J. W.4 aut |
| 700 | 1 | a Johansson, Anders,d 1966-u Umeå universitet,Institutionen för klinisk mikrobiologi,Molekylär Infektionsmedicin, Sverige (MIMS)4 aut0 (Swepub:umu)anjo0135 |
| 700 | 1 | a Colman, R. E.4 aut |
| 700 | 1 | a Schupp, J. M.4 aut |
| 700 | 1 | a Ceyhan, M.4 aut |
| 700 | 1 | a Keim, P. S.4 aut |
| 700 | 1 | a Wagner, D. M.4 aut |
| 710 | 2 | a Umeå universitetb Institutionen för klinisk mikrobiologi4 org |
| 773 | 0 | t BMC Infectious Diseasesd : BMCg 18q 18x 1471-2334 |
| 856 | 4 | u https://doi.org/10.1186/s12879-018-3218-2y Fulltext |
| 856 | 4 | u https://umu.diva-portal.org/smash/get/diva2:1242779/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
| 856 | 4 | u https://bmcinfectdis.biomedcentral.com/track/pdf/10.1186/s12879-018-3218-2 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-150725 |
| 856 | 4 8 | u https://doi.org/10.1186/s12879-018-3218-2 |
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