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Morphological analy...
Morphological analysis of Apolipoprotein E binding to A beta Amyloid using a combination of Surface Plasmon Resonance, Immunogold Labeling and Scanning Electron Microscopy
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- Islam, Tohidul (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Gharibyan, Anna L. (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Lee, Cheng Choo (author)
- Umeå universitet,Kemiska institutionen
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- Olofsson, Anders, 1970- (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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(creator_code:org_t)
- 2019-12-11
- 2019
- English.
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In: BMC Biotechnology. - : BMC. - 1472-6750. ; 19:1
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Abstract
Subject headings
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- Background: Immunogold labeling in combination with transmission electron microscopy analysis is a technique frequently used to correlate high-resolution morphology studies with detailed information regarding localization of specific antigens. Although powerful, the methodology has limitations and it is frequently difficult to acquire a stringent system where unspecific low-affinity interactions are removed prior to analysis.Results: We here describe a combinatorial strategy where surface plasmon resonance and immunogold labeling are used followed by a direct analysis of the sensor-chip surface by scanning electron microscopy. Using this approach, we have probed the interaction between amyloid-beta fibrils, associated to Alzheimer's disease, and apolipoprotein E, a well-known ligand frequently found co-deposited to the fibrillar form of A beta in vivo. The results display a lateral binding of ApoE along the amyloid fibrils and illustrates how the gold-beads represent a good reporter of the binding.Conclusions: This approach exposes a technique with generic features which enables both a quantitative and a morphological evaluation of a ligand-receptor based system. The methodology mediates an advantage compared to traditional immunogold labeling since all washing steps can be monitored and where a high stringency can be maintained throughout the experiment.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology (hsv//eng)
Keyword
- A beta
- ApoE
- Immunogold
- Surface plasmon resonance
- SPR
- Scanning electron microscopy
- SEM
- Fibrils
- Morphology
- Abeta
Publication and Content Type
- ref (subject category)
- art (subject category)
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